May Petra, Schniertshauer Ute, Gerhartz Claudia, Horn Friedemann, Heinrich Peter C
Department of Biochemistry, Universitätsklinikum, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen, Germany.
Acta Biochim Pol. 2003;50(3):595-601.
Interleukin-6 is a potent inducer of acute-phase response gene transcription. The intracellular signal transduction mechanisms by which this and other biological effects of the cytokine are achieved include activation of the JAK-STAT signaling pathway. More specifically, activation of the signal transducers and activators of transcription STAT1, 3, and 5 in response to IL-6 has been described. We examined the relative potency of these three STAT factors for the activation of acute-phase gene promoters in HepG2 cells in a reporter gene-based assay, where specific STAT factors could be activated via recombinant receptor constructs bearing different STAT-recruiting modules. These experiments indicate that amongst the STAT factors known to be activated by IL-6 STAT3 is the most potent activator of acute-phase gene transcription.
白细胞介素-6是急性期反应基因转录的强效诱导剂。细胞因子的这种及其他生物学效应所通过的细胞内信号转导机制包括JAK-STAT信号通路的激活。更具体地说，已经描述了响应白细胞介素-6时转录信号转导子和激活子STAT1、3和5的激活情况。我们在基于报告基因的检测中研究了这三种STAT因子激活HepG2细胞中急性期基因启动子的相对效力，在该检测中，特定的STAT因子可通过携带不同STAT招募模块的重组受体构建体被激活。这些实验表明，在已知被白细胞介素-6激活的STAT因子中，STAT3是急性期基因转录的最有效激活剂。
