Lai C F, Ripperger J, Morella K K, Wang Y, Gearing D P, Horseman N D, Campos S P, Fey G H, Baumann H
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
J Biol Chem. 1995 Oct 6;270(40):23254-7. doi: 10.1074/jbc.270.40.23254.
Transient transfection of expression vectors for various members of the hematopoietin receptor family and STAT proteins into COS-1 cells indicated that each receptor was capable of stimulating the DNA binding activity of STAT1, STAT3, and STAT5B. However, gp130 preferentially activated STAT1 and STAT3. Activation of STAT5B differed from that of the other two in that the box 3 sequence motif in the cytoplasmic domain of gp130 was not required. Moreover, STAT5B and STAT3 enhanced gene transcription via separate regulatory elements. This study has identified two potential signal transduction pathways by which hematopoietin receptors, including the interleukin-6 receptor, control transcription of acute phase plasma protein genes in hepatic cells.
将造血因子受体家族各成员及信号转导和转录激活因子（STAT）蛋白的表达载体瞬时转染至COS-1细胞表明，每种受体都能够刺激STAT1、STAT3和STAT5B的DNA结合活性。然而，gp130优先激活STAT1和STAT3。STAT5B的激活与其他两者不同，因为gp130胞质结构域中的box 3序列基序并非必需。此外，STAT5B和STAT3通过不同的调控元件增强基因转录。本研究确定了两条潜在的信号转导途径，造血因子受体（包括白细胞介素-6受体）可通过这两条途径控制肝细胞中急性期血浆蛋白基因的转录。
