糖皮质激素和盐皮质激素对大鼠心房利钠肽基因表达的体内作用。
Effect of gluco- and mineralocorticoids on gene expression of atrial natriuretic peptide by rat atria in vivo.
作者信息
Hong M, Liu J K, Bao T, Yang S N
机构信息
Department of Biochemistry, Norman Bethune University of Medical Sciences, Changchun.
出版信息
Chin Med J (Engl). 1992 Jul;105(7):549-52.
The effect of gluco- and mineralocorticoids on atrial natriuretic peptide (ANP) gene expression as well as plasma sodium and potassium concentrations was studied in both intact and adrenalectomized Wistar rats. Atria total RNA was extracted with cold phenol method and hybridized to alpha-32P labeled rat ANP-cDNA probe. The ANP gene transcripts in intact rats receiving dexamethasone and in adrenalectomized rats receiving both dexamethasone and deoxy-corticosterone increased by twofold, while there were no significant changes in the other groups. There were no parallel correlations between the rise of corticoid hormones-induced plasma Na+ concentration and the enhancement of rANP-mRNA level. The results suggest that glucocorticoids, when used together with mineralocorticoids, may directly increase ANP gene expression, whereas administration of mineralocorticoids alone has no effect on ANP gene expression.
在完整的和肾上腺切除的Wistar大鼠中，研究了糖皮质激素和盐皮质激素对心房利钠肽（ANP）基因表达以及血浆钠和钾浓度的影响。用冷酚法提取心房总RNA，并与α-32P标记的大鼠ANP - cDNA探针杂交。接受地塞米松的完整大鼠以及接受地塞米松和脱氧皮质酮的肾上腺切除大鼠中的ANP基因转录本增加了两倍，而其他组没有显著变化。皮质激素诱导的血浆Na +浓度升高与rANP - mRNA水平增强之间没有平行相关性。结果表明，糖皮质激素与盐皮质激素一起使用时，可能直接增加ANP基因表达，而单独给予盐皮质激素对ANP基因表达没有影响。
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