利用 LC-(+,-)-ESI-MS 和 LC-PDA 技术对贯叶金丝桃属生物活性成分的去重复：以贯叶连翘为例。
Dereplication of bioactive constituents of the genus hypericum using LC-(+,-)-ESI-MS and LC-PDA techniques: Hypericum triquterifolium as a case study.
机构信息
Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Al Isra Private University, P.O. Box 22, 33, Amman 11622, Jordan.
出版信息
Saudi Pharm J. 2009 Oct;17(4):269-74. doi: 10.1016/j.jsps.2009.10.002. Epub 2009 Nov 10.
Abstract
Utilizing liquid chromatography-electro spray ionization-mass spectrometry (LC-(+,-)-ESI-MS) and liquid chromatography-photodiode array detection (LC-PDA) techniques, a dereplication strategy for the analysis of the secondary metabolites constituents of the genus Hypericum has been developed. From the crude methanolic extract of the aerial parts of H. triquetrifolium (leaves, stems, and flowers) and on the basis of their UV-profiles, chromatographic retention times and (+,-)-ESI-MS (TIC and SIM) mass spectral data, seven known (1-7) compounds were dereplicated fairly rapidly. The compounds were classified into three structural classes: phloroglucinols: hyperfirin and adhyperfirin; naphthodianthrones: hypericin, pseudo-hypericin, proto-hypericin, and protopseudo-hypericin; and the flavonoid rutin.
摘要
利用液相色谱-电喷雾电离-质谱（LC-（+，-）-ESI-MS）和液相色谱-光电二极管阵列检测（LC-PDA）技术，开发了一种用于分析贯叶金丝桃属次生代谢产物成分的去重复策略。从三叶贯叶金丝桃（叶、茎和花）的粗甲醇提取物中，并根据其紫外光谱、色谱保留时间和（+，-）-ESI-MS（TIC 和 SIM）质谱数据，快速鉴定出 7 种已知（1-7）化合物。这些化合物被分为三类结构：苯丙素醇：hyperfirin 和 adhyperfirin；萘二蒽酮：金丝桃素、伪金丝桃素、原金丝桃素和原伪金丝桃素；以及类黄酮芦丁。
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