Liao Chao-Wei, Chiou Hue-Ying, Yeh Kuang-Sheng, Chen Jia-Rong, Weng Chung-Nan
Department of Pathobiology, Pig Research Institute Taiwan, P.O. Box 23, Chu-Nan, Miaoli, Taiwan, ROC.
Prev Vet Med. 2003 Sep 30;61(1):1-15. doi: 10.1016/s0167-5877(02)00195-2.
Oral-vaccine microspheres based on formalin-inactivated Actinobacillus pleuropneumoniae serotype 1 (AP-1) antigens and enteric-coated polymers were prepared using a co-spray drying process. We evaluated using this for a peroral vaccine. We measured specific-antibody titers and protection from challenge in mouse and pig models. In mice (24 per group), a subcutaneous aluminum-adjuvant vaccine or oral vaccination with three doses of AQ6-AP microspheres provided similar protection against intranasal challenge with 5 x 10(8) colony-formation units (cfu) of AP-1 bacterial culture broth. Two weeks after four oral vaccinations with 600 mg of AQ6-AP microsphere acetate solution (containing formalin-inactivated AP-1 antigens of 1.0 x 10(10) cfu bacterial broth), pigs (9 per group) were challenged intranasally with 1 ml of AP-1 bacterial culture broth (5 x 10(9) cfu). The clinical signs, percentage of pig survival ratio, lung lesion areas, and microscopic examinations indicated that the oral AQ6-AP vaccine provided more protection than vaccinating pigs intramuscularly with AP-1 aluminum vaccine.
采用共喷雾干燥工艺制备了基于福尔马林灭活的胸膜肺炎放线杆菌1型（AP-1）抗原和肠溶聚合物的口服疫苗微球。我们评估了将其用于口服疫苗的效果。我们在小鼠和猪模型中测量了特异性抗体滴度以及对攻毒的保护作用。在小鼠（每组24只）中，皮下注射铝佐剂疫苗或口服三剂AQ6-AP微球对用5×10⁸集落形成单位（cfu）的AP-1细菌培养液进行鼻内攻毒提供了相似的保护作用。在用600mg AQ6-AP微球醋酸盐溶液（含有1.0×10¹⁰cfu细菌培养液的福尔马林灭活AP-1抗原）进行四次口服疫苗接种两周后，猪（每组9只）用1ml AP-1细菌培养液（5×10⁹cfu）进行鼻内攻毒。临床症状、猪存活率百分比、肺部病变面积和显微镜检查表明，口服AQ6-AP疫苗比用AP-1铝佐剂疫苗对猪进行肌肉注射提供了更多保护。
