Oishi E, Kitajima T, Koyama Y, Ohgitani T, Katayama S, Okabe T
Division of Veterinary Microbiology, Kyoto Biken Laboratories, Japan.
J Vet Med Sci. 1995 Dec;57(6):1125-8. doi: 10.1292/jvms.57.1125.
Cell-free-antigens prepared from a concentrated culture supernatant of Actinobacillus pleuropneumoniae (A. pleuropneumoniae) serotypes 1, 2 and 5 were mixed and emulsified with oil adjuvant. The combined vaccine of these 3 serotypes of A. pleuropneumoniae was tested for its ability to confer protection. Pigs immunized with the combined vaccine survived and showed no clinical signs against an intratracheal challenge with A. pleuropneumoniae. In contrast, control pigs inoculated with concentrated culture media emulsified with oil adjuvant developed typical symptoms of pleuropneumonia after challenge inoculation.
将从胸膜肺炎放线杆菌（A. pleuropneumoniae）1型、2型和5型浓缩培养上清液中制备的无细胞抗原混合，并用油佐剂乳化。对这3种血清型的胸膜肺炎放线杆菌联合疫苗的保护能力进行了测试。用联合疫苗免疫的猪存活下来，并且在经气管内接种胸膜肺炎放线杆菌后未表现出临床症状。相比之下，接种用油佐剂乳化的浓缩培养基的对照猪在攻毒接种后出现了典型的胸膜肺炎症状。
