Martella Vito, Ciarlet Max, Camarda Antonio, Pratelli Annamaria, Tempesta Maria, Greco Grazia, Cavalli Alessandra, Elia Gabriella, Decaro Nicola, Terio Valentina, Bozzo Giancarlo, Camero Michele, Buonavoglia Canio
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, Italy.
Virology. 2003 Sep 15;314(1):358-70. doi: 10.1016/s0042-6822(03)00418-5.
The genes encoding the glycoprotein VP7, the VP8* trypsin-cleavage product of the protein VP4, a fragment of the protein VP6 associated with subgroup (SG) specificity, and the enterotoxin NSP4 of rotavirus strains identified in diarrheic fecal samples of rabbits in Italy were sequenced. The Italian lapine rotavirus (LRV) strains possessed a G3 VP7, SG I VP6, and KUN-like NSP4, a gene constellation typical of LRVs. One LRV strain (30/96), isolated in 1996, shared the closest amino acid (aa) identity (87-96%) with the P[14] genotype, composed of human and LRV strains. Conversely, three LRV strains (160/01, 229/01, and 308/01), identified in 2001, were highly identical (90-95%) among each other, but showed low aa identity (34-77%) to the VP8* genotype-specific sequences of representative rotavirus strains of all remaining P genotypes. This report confirms the worldwide genetic constellations of LRVs and identifies a novel VP4 genotype in rabbits, tentatively proposed as genotype P[22].
对从意大利兔腹泻粪便样本中鉴定出的轮状病毒株的编码糖蛋白VP7、蛋白VP4的VP8胰蛋白酶切割产物、与亚组（SG）特异性相关的蛋白VP6片段以及肠毒素NSP4的基因进行了测序。意大利兔轮状病毒（LRV）株具有G3 VP7、SG I VP6和KUN样NSP4，这是LRV的典型基因组合。1996年分离出的一株LRV（30/96）与由人和LRV株组成的P[14]基因型具有最接近的氨基酸（aa）同一性（87-96%）。相反，2001年鉴定出的三株LRV（160/01、229/01和308/01）彼此高度相同（90-95%），但与所有其余P基因型代表性轮状病毒株的VP8基因型特异性序列的aa同一性较低（34-77%）。本报告证实了LRV在全球的基因组合，并在兔中鉴定出一种新的VP4基因型，暂定为P[22]基因型。
