对两株美国马轮状病毒毒株和一株英国马轮状病毒毒株的11个基因组片段进行克隆及核苷酸序列分析。
Cloning and nucleotide sequence analyses of 11 genome segments of two American and one British equine rotavirus strains.
作者信息
Ma Yongping, Wen Xiaobo, Hoshino Yasutaka, Yuan L
机构信息
Department of Biochemistry and Molecular Biology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, 400016 Chongqing, China; Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA.
Epidemiology Section, Laboratory of Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Building 50, Room 6308, 50 South Drive, MSC 8026, Bethesda, MD 20892-8026, USA; College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China.
出版信息
Vet Microbiol. 2015 Mar 23;176(1-2):172-8. doi: 10.1016/j.vetmic.2015.01.008. Epub 2015 Jan 15.
Group A equine rotavirus (ERV) is the main cause of diarrhea in foals and causes severe economic loss due to morbidity and mortality on stud farming worldwide. Molecular evolution of equine rotaviruses remains understudies. In this study, whole-genomic analysis of 2 group A ERV, FI-14 (G3P[12]), H-2 (G3P[12]) isolated from American, and FI23 (G14P[12]) from British was carried out and genotype constellations were determined as G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 for FI-14; G14-P[12]-I2-R2-C2-M3-A10-N2-T3-E2-H7 for FI23; and G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7 for H-2, respectively. With the exception of the VP7 and VP6 gene, 2 G3P[12] strains (FI-14 and H-2) and one G14P[12] strain (FI23) were highly related genetically. Of note, the VP6 genotype of H-2 strain was previously reported to be I2, however, sequence and phylogenetic analyses demonstrated that it was I6. Therefore, it showed that G3P[12] ERV strains and G14P[12] ERV strains bore a distinct VP6 genotype: I6 for G3P[12] strains and I2 for G14P[12] strains. Moreover, it demonstrated that T-cell epitope 299P-300P/Q residues (PP/Q) of VP6 may be considered as I2 ERV typical molecular marker, which facilitates the analysis of the molecular evolution of equine rotaviruses.
A组马轮状病毒（ERV）是马驹腹泻的主要病因，在全球种马场中，其导致的发病率和死亡率造成了严重的经济损失。马轮状病毒的分子进化仍在研究之中。在本研究中，对从美国分离出的2株A组ERV（FI-14，G3P[12]；H-2，G3P[12]）以及从英国分离出的FI23（G14P[12]）进行了全基因组分析，并确定其基因组合分别为：FI-14为G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7；FI23为G14-P[12]-I2-R2-C2-M3-A10-N2-T3-E2-H7；H-2为G3-P[12]-I6-R2-C2-M3-A10-N2-T3-E2-H7。除VP7和VP6基因外，2株G3P[12]毒株（FI-14和H-2）和1株G14P[12]毒株（FI23）在基因上高度相关。值得注意的是，H-2毒株的VP6基因型先前报道为I2，但序列和系统发育分析表明它是I6。因此，结果显示G3P[12]ERV毒株和G14P[12]ERV毒株具有不同的VP6基因型：G3P[12]毒株为I6，G14P[12]毒株为I2。此外，研究表明VP6的T细胞表位299P-300P/Q残基（PP/Q）可被视为I2ERV的典型分子标志物，这有助于分析马轮状病毒的分子进化。
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