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将冻融后的小鼠精子注入假孕雌性动物的输卵管后产生正常幼崽。

Production of normal young following insemination of frozen-thawed mouse spermatozoa into fallopian tubes of pseudopregnant females.

作者信息

Nakagata N

机构信息

Nippon Institute for Biological Science, Tokyo, Japan.

出版信息

Jikken Dobutsu. 1992 Oct;41(4):519-22. doi: 10.1538/expanim1978.41.4_519.

DOI:10.1538/expanim1978.41.4_519
PMID:1451761
Abstract

Mouse epididymal spermatozoa in the cryopreservation solution (18% raffinose and 3% skim milk in distilled water) were frozen and stored at -196 degrees C, and later thawed at room temperature. The thawed sperm suspension was inseminated into the Fallopian tubes containing ovulated oocytes in pseudopregnant females on the day of finding the vaginal plug. Five out of 12 females gave birth to 28 Young (5.6 per liter).

摘要

将处于冷冻保存溶液（蒸馏水中含18%棉子糖和3%脱脂乳）中的小鼠附睾精子冷冻并储存在-196℃，随后在室温下解冻。在发现阴道栓当天，将解冻后的精子悬液输精到处于假孕状态的雌性小鼠的输卵管中，输卵管中含有已排卵的卵母细胞。12只雌性小鼠中有5只产下28只幼崽（每窝5.6只）。

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Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected].蛋白二硫键异构酶同源物 PDILT 对于精子膜蛋白 ADAM3 的质量控制和雄性生育力是必需的[更正]。
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