Gomez-Muñoz A, Hatch G M, Martin A, Jamal Z, Vance D E, Brindley D N
Department of Biochemistry, Faculty of Medicine, University of Alberta, Edmonton, Canada.
FEBS Lett. 1992 Apr 13;301(1):103-6. doi: 10.1016/0014-5793(92)80219-7.
Incubation of hepatocytes with okadaic acid displaced the N-ethylmaleimide-sensitive phosphatidate phosphohydrolase from the membrane fraction into the cytosol and partially prevented the oleate-induced movement of phosphohydrolase from cytosol to membranes. However, higher concentrations of oleate still caused translocation and activation of the phosphohydrolase. This enzyme is stimulated by Mg2+, and is probably involved in glycerolipid synthesis. Okadaic acid also decreased the concentration of diacylglycerol within the hepatocytes. Okadiac acid had no observable effect on the activity of an N-ethylmaleimide-insensitive phosphatidate phosphohydrolase which remained firmly attached to membranes. This activity is not stimulated by Mg2+ and is probably involved in signal transduction by the phospholipase D pathway.
用冈田酸孵育肝细胞，可使N - 乙基马来酰亚胺敏感的磷脂酸磷酸水解酶从膜部分转移至胞质溶胶，并部分阻止油酸诱导的磷酸水解酶从胞质溶胶向膜的移动。然而，更高浓度的油酸仍会导致磷酸水解酶的易位和激活。该酶受Mg2 +刺激，可能参与甘油脂质合成。冈田酸还降低了肝细胞内二酰基甘油的浓度。冈田酸对一种N - 乙基马来酰亚胺不敏感的磷脂酸磷酸水解酶的活性没有明显影响，该酶仍牢固地附着在膜上。这种活性不受Mg2 +刺激，可能参与磷脂酶D途径的信号转导。
