Knott R M, Hesketh J E
Rowett Research Institute, Bucksburn, Aberdeen, Scotland, UK.
FEBS Lett. 1992 Sep 7;309(2):153-6. doi: 10.1016/0014-5793(92)81084-y.
Insulin resistance and defective glucose transport are associated with muscle tissue in the genetically obese Zucker rat and are accompanied by changes in the number of insulin receptors and the availability of glucose transporters. The present study was carried out to assess whether, in male Zucker rats at 10 weeks of age, these defects were reflected by changes in the levels of mRNAs for the insulin receptor and for the insulin responsive glucose transporter (GLUT-4). Total RNA was extracted from plantaris and soleus muscles and the levels of insulin receptor and GLUT-4 mRNAs and 18 S rRNA were determined by Northern hybridization and quantified by image analysis of the autoradiographs. A 50% increase in the level of insulin receptor mRNA was detected in both the plantaris and the soleus muscle from the obese rats compared to the lean rats. No change in the level of GLUT-4 mRNA was detected in the plantaris muscle although increases were observed in the soleus muscle from the obese rats.
胰岛素抵抗和葡萄糖转运缺陷与遗传性肥胖的 Zucker 大鼠的肌肉组织有关，并伴随着胰岛素受体数量和葡萄糖转运蛋白可用性的变化。本研究旨在评估 10 周龄雄性 Zucker 大鼠的这些缺陷是否通过胰岛素受体和胰岛素反应性葡萄糖转运蛋白（GLUT-4）的 mRNA 水平变化得以体现。从趾长伸肌和比目鱼肌中提取总 RNA，通过 Northern 杂交测定胰岛素受体和 GLUT-4 mRNA 以及 18S rRNA 的水平，并通过放射自显影片的图像分析进行定量。与瘦大鼠相比，肥胖大鼠的趾长伸肌和比目鱼肌中胰岛素受体 mRNA 水平均检测到 50% 的升高。在趾长伸肌中未检测到 GLUT-4 mRNA 水平的变化，尽管在肥胖大鼠的比目鱼肌中观察到了升高。
