Keller B, Kast P, Hennecke H
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Zürich, Switzerland.
FEBS Lett. 1992 Apr 13;301(1):83-8. doi: 10.1016/0014-5793(92)80215-3.
While crystals suitable for X-ray diffraction analyses are available of phenylalanyl-tRNA synthetase (PheRS) from the thermophilic bacterium Thermus thermophilus, neither the primary structure of its constituent alpha and beta subunits nor the nucleotide sequence of the corresponding pheS and pheT genes were known. Using specific oligonucleotides of conserved pheS regions that were adapted to the T. thermophilus codon usage, we identified, cloned and subsequently sequenced the pheST genes of this bacterium. The sequences reported here will greatly aid in the three-dimensional structure determination of T. thermophilus PheRS, a heterotetrameric (alpha 2 beta 2), class II aminoacyl-tRNA synthetase.
虽然可获得来自嗜热栖热菌的苯丙氨酰 - tRNA合成酶（PheRS）的适合X射线衍射分析的晶体，但其组成的α和β亚基的一级结构以及相应的pheS和pheT基因的核苷酸序列均未知。利用适应嗜热栖热菌密码子使用的保守pheS区域的特异性寡核苷酸，我们鉴定、克隆并随后对该细菌的pheST基因进行了测序。本文报道的序列将极大地有助于嗜热栖热菌PheRS（一种异源四聚体（α2β2）II类氨酰 - tRNA合成酶）的三维结构测定。
