Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes.

The nucleotide sequence of the Bacillus subtilis pheST genes coding for the 2 subunits of phenylalanyl-tRNA synthetase has been determined. The pheS gene corresponds to 1029 bp and the pheT gene to 2412 bp. The encoded proteins have Mrs of 38,947 (343 amino acids, alpha-subunit) and 87,916 (804 amino acids, beta-subunit), respectively. The genes are adjacent on the chromosome separated by only 15 nucleotides. The pheT gene is immediately followed by a hairpin structure typical of a rho-independent transcription terminator. S1 nuclease mapping and primer extension analysis of pheST mRNA revealed a major start of transcription 318 nucleotides upstream of the pheS gene, and 6 nucleotides downstream of a E sigma 43 promoter consensus sequence. Within the 5'-noncoding region several potential secondary structures have been noted.