Brakhage A A, Wozny M, Putzer H
Institut für Mikrobiologie, Universität Münster, Germany.
Biochimie. 1990 Oct;72(10):725-34. doi: 10.1016/0300-9084(90)90157-c.
The nucleotide sequence of the Bacillus subtilis pheST genes coding for the 2 subunits of phenylalanyl-tRNA synthetase has been determined. The pheS gene corresponds to 1029 bp and the pheT gene to 2412 bp. The encoded proteins have Mrs of 38,947 (343 amino acids, alpha-subunit) and 87,916 (804 amino acids, beta-subunit), respectively. The genes are adjacent on the chromosome separated by only 15 nucleotides. The pheT gene is immediately followed by a hairpin structure typical of a rho-independent transcription terminator. S1 nuclease mapping and primer extension analysis of pheST mRNA revealed a major start of transcription 318 nucleotides upstream of the pheS gene, and 6 nucleotides downstream of a E sigma 43 promoter consensus sequence. Within the 5'-noncoding region several potential secondary structures have been noted.
已确定编码苯丙氨酰 - tRNA合成酶2个亚基的枯草芽孢杆菌pheST基因的核苷酸序列。pheS基因对应1029个碱基对，pheT基因对应2412个碱基对。所编码的蛋白质分子量分别为38947（α亚基，343个氨基酸）和87916（β亚基，804个氨基酸）。这些基因在染色体上相邻，仅相隔15个核苷酸。pheT基因紧接着一个典型的不依赖ρ因子的转录终止子的发夹结构。pheST mRNA的S1核酸酶图谱分析和引物延伸分析显示，转录主要起始于pheS基因上游318个核苷酸处，以及E sigma 43启动子共有序列下游6个核苷酸处。在5' - 非编码区内已发现几个潜在的二级结构。
