嗜热栖热菌HB8的tRNA（Phe）的pheU基因的克隆与测序，以及pheU和pheST基因的基因组定位
Cloning and sequencing of the pheU gene for tRNA(Phe) of Thermus thermophilus HB8, and genomic mapping of the pheU and pheST genes.
作者信息
Keller B, Hennecke H
机构信息
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.
出版信息
FEMS Microbiol Lett. 1994 Nov 1;123(3):275-9. doi: 10.1111/j.1574-6968.1994.tb07236.x.
The gene pheU for tRNA(Phe) from the extreme thermophile Thermus thermophilus HB8 was cloned and sequenced. It differed from the published tRNA(Phe) sequence by one nucleotide. Both the pheU gene and the pheST genes encoding the alpha and beta subunits of phenylalanyl-tRNA synthetase were located on the physical map of the T. thermophilus chromosome, where pheU was present on a hitherto unrecognized HpaI DNA fragment.
对嗜热栖热菌HB8的tRNA（Phe）的pheU基因进行了克隆和测序。它与已发表的tRNA（Phe）序列有一个核苷酸的差异。编码苯丙氨酰-tRNA合成酶α和β亚基的pheU基因和pheST基因都位于嗜热栖热菌染色体的物理图谱上，其中pheU存在于一个迄今未被识别的HpaI DNA片段上。
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