Zuo Xinbing, Wang Kemin, Zhou Leiji, Huang Shasheng
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Institute of Biological Technology, Hunan University, Changsha, P. R. China.
Electrophoresis. 2003 Sep;24(18):3202-6. doi: 10.1002/elps.200305596.
Mode-filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 micro m) and a bovine serum albumin (BSA)-modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol-gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT-IR). The separation of DL-tryptophan (10 mM) was investigated under different voltage/current conditions.
通过应用不同尺寸（250、320和530微米）的环形柱以及牛血清白蛋白（BSA）修饰的手性固定相，开发了用于毛细管电色谱（CEC）的模式过滤光毛细管电泳（CE）。使用溶胶-凝胶技术将BSA封装在光纤的预处理表面和毛细管内壁上，从而得到更大的活性固定相面积。通过原子力显微镜（AFM）和傅里叶变换红外光谱（FT-IR）对涂层进行了检测。在不同电压/电流条件下研究了DL-色氨酸（10 mM）的分离情况。
