Stümpel F, Hartmann H
Department of Medicine, Georg-August-Universität, Göttingen, FRG.
Diabetologia. 1992 Oct;35(10):932-8. doi: 10.1007/BF00401421.
The metabolic potency of recombinant human insulin-like growth factor II was studied in anaesthetized adult rats by obtaining dose-response curves for the hypoglycaemic action and for the stimulation of glucose metabolism during euglycaemic clamping. Compared to insulin, about 50 times higher doses of insulin-like growth factor II were required to result in identical in vivo responses, with half-maximally effective serum concentrations for the stimulation of glucose disposal during clamp studies of about 0.8 and 50 pmol/ml, respectively. A similar difference in potency was observed for the dose-dependent stimulatory actions on glucose metabolism in individual target tissues. Half-maximally effective serum concentrations in the range of 0.8 to 3.0 pmol/ml for insulin and of 40 to 70 pmol/ml for insulin-like growth factor II were seen to be required for 2-deoxyglucose uptake, glycogen formation in skeletal muscle and lipogenesis in epididymal fat. Maximal responses were identical with both peptides. These data suggest that in vivo acute metabolic actions of insulin-like growth factor II on carbohydrate metabolism occurred through insulin receptors.
通过获取麻醉成年大鼠在正常血糖钳夹期间低血糖作用和葡萄糖代谢刺激的剂量反应曲线，研究了重组人胰岛素样生长因子II的代谢效力。与胰岛素相比，需要大约高50倍剂量的胰岛素样生长因子II才能产生相同的体内反应，在钳夹研究期间刺激葡萄糖处置的半数有效血清浓度分别约为0.8和50 pmol/ml。在各个靶组织中，对葡萄糖代谢的剂量依赖性刺激作用也观察到类似的效力差异。对于2-脱氧葡萄糖摄取、骨骼肌糖原形成和附睾脂肪脂肪生成，胰岛素的半数有效血清浓度在0.8至3.0 pmol/ml范围内，胰岛素样生长因子II的半数有效血清浓度在40至70 pmol/ml范围内。两种肽的最大反应相同。这些数据表明，胰岛素样生长因子II对碳水化合物代谢的体内急性代谢作用是通过胰岛素受体发生的。
