Häsler Julien, Wüest Jean, Gaspar Thomas, Crèvecoeur Michèle
Laboratoire de biochimie et physiologie végétales, université de Genève, place de l'Université 3, 1211 4 Genève, Suisse.
Biol Cell. 2003 Sep;95(6):357-64. doi: 10.1016/s0248-4900(03)00077-7.
Cells from a green normal (dependent on exogenous hormones) callus and from an achlorophyllous fully habituated (independent from exogenous hormones) callus, both generated from the same sugarbeet strain more than twenty years ago, were reexamined cytologically, ten years after the first comparative description. Cells from the habituated callus, already considered as neoplastic cells, because terminating a neoplastic progression where the organogenic totipotency was lost, still showed nuclear invaginations, polynucleolation, vacuolation of nucleoli and incomplete cell walls, nevertheless at a higher degree. The present study particularly shows that, compared to their previous description, normal cells have started to acquire some features (polynucleolation, nuclear invaginations.) that are typical of the neoplastic cells. This suggests that normal cells, on the long term, also entered a neoplastic progression, which should explain the known progressive loss of regeneration capacity of too long subcultured hormone-dependent calli.
来自绿色正常（依赖外源激素）愈伤组织和无叶绿素的完全驯化（不依赖外源激素）愈伤组织的细胞，这两种愈伤组织均由二十多年前的同一甜菜品系产生，在首次进行比较描述十年后，对其进行了细胞学重新检查。来自驯化愈伤组织的细胞，已被视为肿瘤细胞，因为其终止了器官发生全能性丧失的肿瘤进展，仍表现出核内陷、多核仁、核仁空泡化和不完全细胞壁，不过程度更高。本研究特别表明，与之前的描述相比，正常细胞已开始获得一些肿瘤细胞典型的特征（多核仁、核内陷）。这表明正常细胞从长期来看也进入了肿瘤进展过程，这应该可以解释长期继代培养的激素依赖性愈伤组织再生能力逐渐丧失的现象。
