Martínez L D, Noher de Halac I
Centro de Microscopía Electrónica, Universidad Católica de Córdoba, Obispo Trejo 323, (5000) Córdoba, Argentina.
Biocell. 2000 Dec;24(3):239-46.
Anther culture of O. hookeri on Murashige and Skoog (1962) medium supplemented with 2 mg l-1 2,4-dichlorophenoxyacetic acid and 2 mg-1 1-naphthaleneacetic acid produced callus formation. When subcultured onto medium lacking auxin, the callus regenerated through the organogenic pathway. Non-organogenic and organogenic callus was observed using histological methods after 2, 3 and 24 weeks in culture. Three types of calli were recognized: non-organogenic friable calli, organogenic friable calli with roots and organogenic hard calli with shoots. The microscopical sections showed striking differences in tissue organization among friable and compact calli. Vascular bundles were prominent in compact calli, but were not found in friable calli. Calli sections showed at light microscopy cells at two developmental stages; differentiated highly vacuolated cells and meristematic small isodiametric cells with densely stained cytoplasm. At electron microscopy level abnormal chloroplasts were present in non-organogenic calli, while chloroplasts were well developed in organogenic hard calli. Peroxisomes with paracrystalline protein bodies were abundant in both types of calli.
在添加了2毫克/升2,4 - 二氯苯氧乙酸和2毫克/升1 - 萘乙酸的Murashige和Skoog（1962）培养基上对胡克稻进行花药培养，产生了愈伤组织。当转接至不含生长素的培养基上时，愈伤组织通过器官发生途径再生。在培养2周、3周和24周后，使用组织学方法观察到了非器官发生型和器官发生型愈伤组织。识别出了三种类型的愈伤组织：非器官发生型易碎愈伤组织、有根的器官发生型易碎愈伤组织和有芽的器官发生型坚硬愈伤组织。显微镜切片显示易碎愈伤组织和致密愈伤组织在组织结构上有显著差异。致密愈伤组织中维管束明显，但易碎愈伤组织中未发现。愈伤组织切片在光学显微镜下显示处于两个发育阶段的细胞；分化的高度液泡化细胞和具有密集染色细胞质的分生小等径细胞。在电子显微镜水平上，非器官发生型愈伤组织中存在异常叶绿体，而器官发生型坚硬愈伤组织中的叶绿体发育良好。两种类型的愈伤组织中都富含带有类晶体蛋白体的过氧化物酶体。
