Yoshioka Mayumi, Tanaka Hiroaki, Shono Naoko, Snyder Eric E, Shindo Munehiro, St-Amand Jonny
Molecular Endocrinology and Oncology Research Center, Laval University Medical Center (CHUL) and Laval University, 2705 Boul. Laurier, Ste-Foy, Québec G1V 4G2, Canada.
FASEB J. 2003 Oct;17(13):1812-9. doi: 10.1096/fj.02-1200com.
Physical exercise produces several adaptive changes in skeletal muscle. However, the molecular mechanisms of these effects are poorly understood. We performed serial analysis of gene expression (SAGE) to quantify the global gene expression profile in sedentary and endurance-trained muscle. A total of 10869 SAGE tags was sequenced and represented 4727 genes. The genes most expressed in muscle are mainly involved in contraction and energy metabolism. Thirty-three genes were differentially expressed between endurance athletes and sedentary individuals. Four genes such as myosin binding protein C fast-type, glycogen phosphorylase, and pyruvate kinase were expressed less in endurance athletes, whereas eight genes coding for expressed sequence tag similar to (EST) crystallin alpha B, EST myosin light chain 2, EST surfactant pulmonary-associated protein A1, EST thrombospondin, EST fructose-bisphosphate aldolase A, EST cytochrome oxidase 1, NADH dehydrogenase 3, and G8 protein were up-regulated. Most of the up-regulated tags corresponded to novel genes. On the other hand, different isoforms of fructose-bisphosphate aldolase A were also differentially expressed. The current study underlying the most highly expressed genes allows a better understanding of global muscle characteristics in normal and endurance-trained individuals. Moreover, the current data suggest novel candidate genes that may be responsible for enhanced endurance performance.
体育锻炼会使骨骼肌产生多种适应性变化。然而，这些效应的分子机制却鲜为人知。我们进行了基因表达系列分析（SAGE），以量化久坐不动和耐力训练肌肉中的整体基因表达谱。总共对10869个SAGE标签进行了测序，代表了4727个基因。在肌肉中表达最多的基因主要参与收缩和能量代谢。耐力运动员和久坐不动的个体之间有33个基因存在差异表达。诸如快肌型肌球蛋白结合蛋白C、糖原磷酸化酶和丙酮酸激酶等4个基因在耐力运动员中的表达较少，而8个与（EST）αB晶状体蛋白、EST肌球蛋白轻链2、EST肺表面活性物质相关蛋白A1、EST血小板反应蛋白、EST果糖二磷酸醛缩酶A、EST细胞色素氧化酶1、NADH脱氢酶3和G8蛋白相似的表达序列标签编码的基因上调。大多数上调的标签对应于新基因。另一方面，果糖二磷酸醛缩酶A的不同同工型也存在差异表达。目前关于表达最高的基因的研究有助于更好地了解正常人和耐力训练个体的整体肌肉特征。此外，目前的数据表明了可能与耐力表现增强有关的新候选基因。
