Nozaki Hiroyuki, Suzuki Shun-ichi, Tsuyoshi Naoko, Yokozeki Kenzo
Aminoscience Laboratories, Ajinomoto Co., Ltd., Kawasaki, Japan.
Biosci Biotechnol Biochem. 2003 Sep;67(9):1923-9. doi: 10.1271/bbb.67.1923.
A potent producer of D-arabitol was isolated by screening of natural sources and identified as Metschnikowia reukaufii AJ14787. Resting cells of this strain can efficiently produce D-arabitol from D-glucose with a weight yield of more than 60%, and can also produce D-arabitol from several other types of sugars such as polyols, ketoses, and aldoses. To improve productivity, various culture conditions such as temperature and the concentrations of D-glucose and nitrogen sources were examined. Under optimal conditions, 206 g/l of D-arabitol was produced from D-glucose with a weight yield of 52% in 100 hours.
通过对天然来源进行筛选，分离出了一种高效生产D-阿拉伯糖醇的菌株，并鉴定为鲁考夫梅奇酵母AJ14787。该菌株的静止细胞能够以超过60%的重量产率从D-葡萄糖高效生产D-阿拉伯糖醇，并且还能从多种其他类型的糖（如多元醇、酮糖和醛糖）生产D-阿拉伯糖醇。为了提高生产率，研究了各种培养条件，如温度以及D-葡萄糖和氮源的浓度。在最佳条件下，100小时内从D-葡萄糖生产出了206 g/l的D-阿拉伯糖醇，重量产率为52%。
