Fan Ting-Ting, Chen Chao, Zeng Du-Wen, Wang Feng-Lou, Xu Zhao-Xian, Jin Ming-Jie, Zou Yue, Li Jun, Zhao Xin-Qing
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
College of Life Science, Dalian Minzu University, Dalian 116600, China.
J Fungi (Basel). 2024 Dec 31;11(1):20. doi: 10.3390/jof11010020.
γ-Aminobutyric acid (GABA) is a valuable amino acid widely used in food, healthcare, and agriculture. GABA bioproduction by budding yeasts has been commonly reported, but related studies using non-conventional yeasts remain limited. In this study, two non-conventional natural yeast strains, namely, JMY140K and JMY075, were identified as promising GABA producers, and JMY075 was discovered to be a GABA producer. Enhanced GABA production was observed in the two yeast strains under stress conditions, including high temperature and high ethanol and acetic acid levels. In particular, JMY140K showed 7.93 times higher GABA titers under thermal stress than that of the control. External stress conditions significantly influenced the GABA production of these two yeast strains. The culture filtrate of JMY140K also showed promising activities in human skin cells. In addition, JMY140K could also produce GABA using rice straw hydrolysate, which indicated that it has the potential to produce GABA using renewable biomass. Our studies provide insight for further enhancing the GABA production of natural yeasts and promoting its biotechnology applications.
γ-氨基丁酸（GABA）是一种有价值的氨基酸，广泛应用于食品、医疗保健和农业领域。通过出芽酵母进行GABA生物合成的报道较为常见，但使用非常规酵母的相关研究仍然有限。在本研究中，两种非常规天然酵母菌株JMY140K和JMY075被鉴定为有潜力的GABA生产者，并且发现JMY075是一种GABA生产者。在高温、高乙醇和高乙酸水平等应激条件下，观察到这两种酵母菌株的GABA产量有所提高。特别是，JMY140K在热应激条件下的GABA滴度比对照高7.93倍。外部应激条件显著影响这两种酵母菌株的GABA产量。JMY140K的培养滤液在人皮肤细胞中也显示出有前景的活性。此外，JMY140K还可以利用稻草水解产物生产GABA，这表明它有利用可再生生物质生产GABA的潜力。我们的研究为进一步提高天然酵母的GABA产量及其生物技术应用提供了见解。
