担子菌真菌杨树菇中编码二氢乳清酸脱氢酶的URA1基因序列。
Sequence of the URA1 gene encoding dihydroorotate dehydrogenase from the basidiomycete fungus Agrocybe aegerita.
作者信息
Noël T, Labarère J
机构信息
Laboratoire de Génétique Moléculaire et Amelioration des Champignons Cultivés, Université de Bordeaux II-INRA, Villenave d'Ornon, France.
出版信息
Gene. 1992 Dec 1;122(1):233-4. doi: 10.1016/0378-1119(92)90057-v.
The URA1 gene encoding dihydroorotate dehydrogenase (DHOdehase) from the edible basidiomycete, Agrocybe aegerita, has been cloned by complementation of the Escherichia coli pyrD mutation. The nucleotide sequence of a 1531-bp genomic fragment carrying URA1 revealed two uninterrupted open reading frames (ORFs) separated by 61 bp. The larger ORF can encode a 328-amino acid (aa) DHOdehase that has 53% homology with the corresponding protein from E. coli. Comparison with other DHOdehase aa sequences showed essentially conservation of the cofactor-binding site of flavoproteins.
通过对大肠杆菌pyrD突变体进行互补，克隆出了来自可食用担子菌盖囊侧耳的编码二氢乳清酸脱氢酶（DHOdehase）的URA1基因。携带URA1的1531 bp基因组片段的核苷酸序列显示有两个不间断的开放阅读框（ORF），间隔61 bp。较大的ORF可编码一个328个氨基酸的DHOdehase，它与来自大肠杆菌的相应蛋白质有53%的同源性。与其他DHOdehase氨基酸序列的比较表明，黄素蛋白的辅因子结合位点基本保守。
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