Khalturin M D, Litvinchuk S N, Borkin L Ia, Rozanov Iu M, Mil'to K D
Institute of Cytology RAS, St. Petersburg.
Tsitologiia. 2003;45(3):308-23.
Genetic differences (presumed 23 loci) between two cryptic forms of Pelobates fuscus, differing in genome size, were examined by means of polyacrylamide-gel electrophoresis. This method allowed to discriminate between these forms. Average genetic distance (DN) between both the forms was equal to 0.311, ranging from 0.055 to 0.563. As a rule, differences within these groups were smaller (0.021-0.388). The data show obvious genetic differentiation between these two cryptic forms of P. fuscus. Differences between these forms and P. syriacus were significantly higher (in average 0.943). Genetic distances in relation to speciation and species concepts are discussed.
通过聚丙烯酰胺凝胶电泳研究了两种基因组大小不同的隐存型棕蟾蜍(Pelobates fuscus)之间的遗传差异(推测有23个基因座)。该方法能够区分这些类型。两种类型之间的平均遗传距离(DN)等于0.311,范围在0.055至0.563之间。通常,这些组内的差异较小(0.021 - 0.388)。数据显示这两种隐存型棕蟾蜍之间存在明显的遗传分化。这些类型与叙利亚蟾蜍(P. syriacus)之间的差异明显更高(平均为0.943)。讨论了与物种形成和物种概念相关的遗传距离。