Terpstra W J, Schoone G J, ter Schegget J
J Med Microbiol. 1986 Aug;22(1):23-8. doi: 10.1099/00222615-22-1-23.
Dot hybridisation with 32P- and biotin-labelled probes prepared from leptospiral DNA was performed to develop a sensitive and specific diagnostic method for early infection with leptospires. The smallest amounts of leptospiral DNA that could be detected with 32P- and biotin-labelled probes was 1.5 pg and 5 pg, respectively, corresponding to about 750 and 2500 leptospires. Dot hybridisation with a 32P-labelled probe detected leptospiral DNA in sera from all of 14 experimentally infected golden hamsters. The smallest amount of leptospiral DNA detected in these experiments corresponded to about 2500 leptospires. In the test conditions described in this study, the sensitivity of dot hybridisation with a biotin-labelled probe was lower. Little cross-hybridisation was observed with unrelated DNAs which indicates that dot hybridisation could be a useful diagnostic method.
为开发一种灵敏且特异的钩端螺旋体早期感染诊断方法，进行了用从钩端螺旋体DNA制备的32P和生物素标记探针的斑点杂交。用32P和生物素标记探针能检测到的钩端螺旋体DNA的最小量分别为1.5 pg和5 pg，分别对应约750个和2500个钩端螺旋体。用32P标记探针的斑点杂交在14只实验感染的金黄仓鼠的所有血清中均检测到钩端螺旋体DNA。这些实验中检测到的钩端螺旋体DNA的最小量对应约2500个钩端螺旋体。在本研究描述的测试条件下，用生物素标记探针的斑点杂交灵敏度较低。未观察到与无关DNA的交叉杂交，这表明斑点杂交可能是一种有用的诊断方法。
