Cabrera T, Garrido V, Concha A, Martín J, Esquivias J, Oliva M R, Ruiz-Cabello F, Serrano S, Garrido F
Servicio de Anális Clínicos e Inmunología, Hospital Virgen de las Nieves, Granada, Spain.
Immunobiology. 1992 Sep;185(5):440-52. doi: 10.1016/s0171-2985(11)80086-0.
Fifty basal cell carcinomas (BCC) and 8 samples of healthy skin were studied for HLA class I and class II antigen expression and for the presence of mutations in codon 12 of the K-ras and H-ras genes. All samples of healthy skin and of epithelium near the tumor showed high levels of class I molecules, whereas 38% of the tumors showed complete absence. Sixty-two percent of the tumors presented positive class I expression with heterogeneous staining. These losses were due to the simultaneous lack of heavy chain and beta 2-microglobulin. Selective losses of HLA-A or HLA-B antigens were not detected. Class II antigens were absent in most of the tumors, only two tumors showing a few weakly positive cells with anti-HLA-DR mAb. The loss of class I expression correlated significantly with the degree of histological differentiation and aggressiveness. We were unable to correlate class I expression with clinical size, depth of invasion or the extent of leukocytic infiltrate surrounding the tumor. Analysis by PCR amplification of codon 12 of the K-ras and H-ras oncogenes detected H-ras mutations in 1 out of 50 cases, and no K-ras mutations in any of the tumors studied. Thus, a positive relationship between K-ras and H-ras mutations and BCC tumorigenesis or MHC alterations seems unlikely in this neoplasia.
对50例基底细胞癌（BCC）和8份健康皮肤样本进行了研究，检测其HLAⅠ类和Ⅱ类抗原表达以及K-ras和H-ras基因第12密码子的突变情况。所有健康皮肤样本及肿瘤附近的上皮组织均显示高水平的Ⅰ类分子，而38%的肿瘤显示完全缺失。62%的肿瘤呈现Ⅰ类抗原阳性表达，但染色不均一。这些缺失是由于重链和β2-微球蛋白同时缺乏所致。未检测到HLA-A或HLA-B抗原的选择性缺失。大多数肿瘤中Ⅱ类抗原缺失，仅有2例肿瘤显示少数抗HLA-DR单克隆抗体弱阳性细胞。Ⅰ类抗原表达的缺失与组织学分化程度和侵袭性显著相关。我们未能将Ⅰ类抗原表达与临床大小、浸润深度或肿瘤周围白细胞浸润程度相关联。通过对K-ras和H-ras癌基因第12密码子进行PCR扩增分析，在50例病例中检测到1例H-ras突变，在所研究的任何肿瘤中均未检测到K-ras突变。因此，在这种肿瘤中，K-ras和H-ras突变与BCC肿瘤发生或MHC改变之间似乎不太可能存在正相关关系。
