Ganapathy K, Bradbury J M
Department of Veterinary Pathology, University of Liverpool, Leahurst, Neston, South Wirral, CH64 7TE, UK.
Avian Pathol. 2003 Oct;32(5):495-502. doi: 10.1080/0307945031000154099.
Immune responses to the virulent S6 strain of Mycoplasma gallisepticum in immunocompetent and cyclosporin A (CsA)-treated specific pathogen free chickens were investigated, and pathogenesis of the M. gallisepticum strain was also examined. Ten-day-old specific pathogen free chickens were inoculated by eye-drop with M. gallisepticum, and a control uninfected group was inoculated with mycoplasma broth. Blood was collected weekly for 4 weeks from five birds in each group and whole blood lymphocyte transformation assayed against concanavalin A and lipopolysaccharide. Blood samples were also collected at intervals for serological assays. Live body weight, clinical signs and lesions were monitored, and recovery of M. gallisepticum was attempted from choanal cleft of live birds and also from various sites at necropsy. In parallel to the aforementioned groups, another set of two groups of chicks treated with CsA was infected with M. gallisepticum S6 or mycoplasma broth. These groups were subjected to the same experimental procedures. In the immunocompetent chickens, M. gallisepticum caused temporary T-cell suppression at 2 weeks post-infection. Comparison of the clinical signs and macroscopic lesions produced in immunocompetent and CsA-treated chickens indicated that T cells may not play an active role in disease development. The percentage of birds with mycoplasma isolation and the load of mycoplasmas suggested that T cells may have some role in resisting mycoplasma colonization or in the elimination of the infection.
研究了免疫活性和环孢菌素A（CsA）处理的无特定病原体鸡对鸡毒支原体强毒株S6的免疫反应，并检测了该鸡毒支原体菌株的发病机制。10日龄无特定病原体鸡通过滴眼接种鸡毒支原体，未感染的对照组接种支原体肉汤。每组每周从5只鸡采集血液，共采集4周，检测全血淋巴细胞对刀豆球蛋白A和脂多糖的转化情况。还定期采集血样进行血清学检测。监测活体重、临床症状和病变，并尝试从活禽后鼻孔裂隙以及尸检时的各个部位分离鸡毒支原体。与上述组平行，另一组两组用CsA处理的雏鸡感染鸡毒支原体S6或支原体肉汤。这些组接受相同的实验程序。在免疫活性鸡中，鸡毒支原体在感染后2周引起暂时的T细胞抑制。对免疫活性鸡和CsA处理鸡产生的临床症状和宏观病变的比较表明，T细胞可能在疾病发展中不发挥积极作用。支原体分离阳性鸡的百分比和支原体载量表明，T细胞可能在抵抗支原体定植或消除感染方面发挥一定作用。
