Reinhardt Anita K, Gautier-Bouchardon Anne V, Gicquel-Bruneau Mireille, Kobisch Marylène, Kempf Isabelle
Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Laboratoire d'Etudes et de Recherches Avicoles et Porcines, Unité de Mycoplasmologie-Bactériologie, BP 53, 22440 Ploufragan, France.
Vet Microbiol. 2005 Mar 20;106(1-2):129-37. doi: 10.1016/j.vetmic.2004.11.016. Epub 2005 Jan 24.
The ability of the avian pathogen Mycoplasma gallisepticum to persist despite fluoroquinolone treatment was investigated in chickens. Groups of specific pathogen free chickens were experimentally infected with M. gallisepticum and treated with enrofloxacin at increasing concentrations up to the therapeutic dose. When M. gallisepticum could no longer be re-isolated from chickens, birds were stressed by inoculation of infectious bronchitis virus or avian pneumovirus. Although M. gallisepticum could not be cultured from tracheal swabs collected on several consecutive sampling days after the end of the enrofloxacin treatments, the infection was not eradicated. Viral infections reactivated the mycoplasma infection. Mycoplasmas were isolated from tracheal rings cultured for several days, suggesting that M. gallisepticum persisted in the trachea despite the enrofloxacin treatment. The minimal inhibitory concentration (MIC) of enrofloxacin for most of the re-isolated mycoplasmas was the same as that of the strain with which the birds were inoculated. Furthermore, no mutation could be detected in the fluoroquinolone target genes. These results suggest that M. gallisepticum can persist in chickens without development of resistance despite several treatments with enrofloxacin.
研究人员在鸡身上研究了禽病原体鸡毒支原体在接受氟喹诺酮治疗后仍能持续存在的能力。将无特定病原体的鸡群实验性感染鸡毒支原体，并用浓度不断增加直至治疗剂量的恩诺沙星进行治疗。当从鸡身上再也无法重新分离出鸡毒支原体时，通过接种传染性支气管炎病毒或禽肺炎病毒对鸡施加应激。尽管在恩诺沙星治疗结束后的连续几天采集的气管拭子中无法培养出鸡毒支原体，但感染并未根除。病毒感染使支原体感染重新激活。从培养了几天的气管环中分离出了支原体，这表明尽管使用了恩诺沙星治疗，鸡毒支原体仍在气管中持续存在。大多数重新分离出的支原体对恩诺沙星的最低抑菌浓度（MIC）与接种鸡的菌株相同。此外，在氟喹诺酮靶基因中未检测到突变。这些结果表明，尽管用恩诺沙星进行了多次治疗，但鸡毒支原体仍可在鸡体内持续存在而不产生耐药性。
