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人血清中p60抗体的鉴定以及通过HlyB-HlyD分泌系统在减毒沙门氏菌表面呈递这种李斯特菌抗原。

Identification of p60 antibodies in human sera and presentation of this listerial antigen on the surface of attenuated salmonellae by the HlyB-HlyD secretion system.

作者信息

Gentschev I, Sokolovic Z, Köhler S, Krohne G F, Hof H, Wagner J, Goebel W

机构信息

Lehrstuhl für Mikrobiologie, Theodor-Boveri-Institut für Biowissenschaften (Biozentrum), Universität Würzburg, Germany.

出版信息

Infect Immun. 1992 Dec;60(12):5091-8. doi: 10.1128/iai.60.12.5091-5098.1992.

DOI:10.1128/iai.60.12.5091-5098.1992
PMID:1452342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC258282/
Abstract

Antibodies directed against the major secreted protein of Listeria monocytogenes, termed p60, were found more frequently than antilisteriolysin antibodies in sera of listeriosis patients. Anti-p60 antibodies were also identified in all tested sera from healthy individuals. To test whether p60 provides protection against L. monocytogenes, we constructed an attenuated Salmonella typhimurium aroA strain which secretes p60 via the Escherichia coli hemolysin secretion pathway. Application of this Salmonella strain to BALB/c mice prior to an L. monocytogenes infection induced p60 antibodies in these mice and led to a significantly reduced number of viable bacteria in the spleen compared with that in control animals which were primed with the S. typhimurium aroA strain alone.

摘要

在李斯特菌病患者血清中，针对单核细胞增生李斯特菌主要分泌蛋白（称为p60）的抗体比抗李斯特菌溶血素抗体更常见。在所有来自健康个体的测试血清中也鉴定出了抗p60抗体。为了测试p60是否能提供针对单核细胞增生李斯特菌的保护作用，我们构建了一株减毒鼠伤寒沙门氏菌aroA菌株，该菌株通过大肠杆菌溶血素分泌途径分泌p60。在单核细胞增生李斯特菌感染之前，将这种沙门氏菌菌株应用于BALB/c小鼠，可诱导这些小鼠产生p60抗体，与仅用鼠伤寒沙门氏菌aroA菌株进行免疫的对照动物相比，其脾脏中活菌数量显著减少。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/3b74b35333cd/iai00036-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/2d128b345fff/iai00036-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/2a6e6acdfd45/iai00036-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/0aee5794669a/iai00036-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/3b74b35333cd/iai00036-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/2d128b345fff/iai00036-0127-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/2a6e6acdfd45/iai00036-0128-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/0aee5794669a/iai00036-0129-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38b9/258282/3b74b35333cd/iai00036-0130-a.jpg

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