Upadhyay S N, Dhawan S, Garg S, Talwar G P
National Institute of Immunology, New Delhi, India.
Int J Immunopharmacol. 1992 Oct;14(7):1187-93. doi: 10.1016/0192-0561(92)90054-o.
Immunomodulatory effects of neem oil were studied in mice. The animals were treated intraperitoneally (i.p.) with neem oil; control animals received the emulsifying agent with or without peanut oil. Peritoneal lavage, collected on subsequent days, showed a maximum number of leukocytic cells on day 3 following treatment with neem oil; peritoneal macrophages exhibited enhanced phagocytic activity and expression of MHC class-II antigens. Neem oil treatment also induced the production of gamma interferon. Spleen cells of neem oil-treated animals showed a significantly higher lymphocyte proliferative response to in vitro challenge with Con A or tetanus toxoid (TT) than that of the controls. Pre-treatment with neem oil, however, did not augment the anti-TT antibody response. The results of this study indicate that neem oil acts as a non-specific immunostimulant and that it selectively activates the cell-mediated immune (CMI) mechanisms to elicit an enhanced response to subsequent mitogenic or antigenic challenge.
研究了印楝油对小鼠的免疫调节作用。给动物腹腔注射印楝油；对照动物接受含有或不含花生油的乳化剂。在随后几天收集的腹腔灌洗液显示，用印楝油处理后第3天白细胞数量最多；腹腔巨噬细胞表现出增强的吞噬活性和MHC-II类抗原的表达。印楝油处理还诱导了γ干扰素的产生。与对照组相比，用印楝油处理的动物的脾细胞对刀豆球蛋白A或破伤风类毒素（TT）的体外刺激表现出显著更高的淋巴细胞增殖反应。然而，用印楝油预处理并没有增强抗TT抗体反应。这项研究的结果表明，印楝油作为一种非特异性免疫刺激剂，它选择性地激活细胞介导的免疫（CMI）机制，以引发对随后的有丝分裂原或抗原刺激的增强反应。
