Tabata T, Watanabe W, Horita K, Kamegai J, Moriyama S, Hibino Y, Ohashi Y, Sugano N
Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan.
Immunopharmacology. 1992 Jul-Aug;24(1):57-63. doi: 10.1016/0162-3109(92)90070-s.
The water soluble material (LEM) was prepared from the solid culture medium in which Lentinus edodes mycelia were growing actively. An alcohol insoluble material was prepared from LEM and subjected to Sepharose 6B gel filtration. The void fraction (LAP1) was composed mainly of xylose-rich heteroglycan and protein. From LAP1, a heteroglycan fraction (LAF1) was prepared by DEAE-Sepharose CL-6B column chromatography. LAP1 and LAF1 enhanced the incorporation of [3H]thymidine into mouse splenic cells (SPs). At each of the optimum doses, the rate of the incorporation was about 10 times as high with LAF1 as with LAP1. Such mitogenic responses were not induced in nylon-column effluent SPs and thymocytes. sIg-expressed cells were responsive to LAF1, but not to LAP1. Moreover, with each fraction, the incorporation was enhanced more in plastic adherent splenic cells (ADs) than in SPs. The flow cytometric assay revealed that the number of Mac-1+ cells is about 13 times as many in ADs as in SPs and that the number of Ly-5+ or Thy-1.2+ cells is considerably reduced in ADs compared with that in SPs. Thus, the present studies suggest that LAP1 and LAF1 act as mitogens predominantly for mouse splenic macrophages and/or monocytes.
水溶性物质（LEM）由香菇菌丝体活跃生长的固体培养基制备而成。从LEM中制备出一种醇不溶性物质，并对其进行琼脂糖6B凝胶过滤。空体积部分（LAP1）主要由富含木糖的杂聚糖和蛋白质组成。从LAP1中，通过DEAE-琼脂糖CL-6B柱色谱法制备出杂聚糖部分（LAF1）。LAP1和LAF1增强了[3H]胸苷掺入小鼠脾细胞（SPs）的能力。在每个最佳剂量下，LAF1的掺入率约为LAP1的10倍。这种促有丝分裂反应在尼龙柱流出的SPs和胸腺细胞中未被诱导。表面免疫球蛋白（sIg）表达细胞对LAF1有反应，但对LAP1无反应。此外，对于每个部分，塑料贴壁脾细胞（ADs）中的掺入增强程度均高于SPs。流式细胞术分析显示，ADs中Mac-1+细胞的数量约为SPs中的13倍，且与SPs相比，ADs中Ly-5+或Thy-1.2+细胞的数量显著减少。因此，本研究表明LAP1和LAF1主要作为小鼠脾巨噬细胞和/或单核细胞的促有丝分裂原。
