Sugano N, Choji Y, Hibino Y, Yasumura S, Maeda H
Cancer Lett. 1985 May;27(1):1-6. doi: 10.1016/0304-3835(85)90002-3.
From the culture medium of Lentinus edodes mycelia, water-soluble material (LEM) was prepared and further fractionated by alcohol precipitation and gel filtration on Sepharose 6B. The resulting fraction of xylose-rich proteoglycan at the void volume was designated as LAP1. The 25% and 50% survival rates of hepatoma-bearing rats were raised by intraperitoneal (i.p.) administration of LAP1 at doses of 3-10 mg/kg (an optimum dose, 3 mg/kg). This fraction did not suppress in vitro cell proliferation of the hepatoma. Moreover, the i.p. administration of LAP1 significantly augmented the activity of macrophage-migration inhibition of the splenic cells from hepatoma-bearing rats in the early stage after transplantation. Thus, the anticarcinogenic action of LAP1 would partly be interpreted by host-dependent immunomodulation.
从香菇菌丝体的培养基中制备了水溶性物质（LEM），并通过乙醇沉淀和Sepharose 6B凝胶过滤进一步分级分离。在空体积处得到的富含木糖的蛋白聚糖级分被命名为LAP1。通过以3-10mg/kg的剂量腹腔注射（i.p.）LAP1（最佳剂量为3mg/kg），提高了荷肝癌大鼠的25%和50%存活率。该级分不抑制肝癌细胞的体外增殖。此外，在移植后早期，腹腔注射LAP1显著增强了荷肝癌大鼠脾细胞的巨噬细胞迁移抑制活性。因此，LAP1的抗癌作用部分可通过宿主依赖性免疫调节来解释。
