Pallister C J, Johnson E M, Warnock D W, Elliot P J, Reeves D F
Department of Biological Sciences, Bristol Polytechnic, UK.
J Antimicrob Chemother. 1992 Sep;30(3):313-20. doi: 10.1093/jac/30.3.313.
Liposomal amphotericin B (AmBisome), at concentrations > or = 20 mg/L, and amphotericin B-deoxycholate (DC) (Fungizone), at concentrations > or = 1 mg/L, both caused a significant reduction in neutrophil uptake of Candida albicans blastospores following simultaneous addition of the drug with the blastospores. The reduction in uptake was seen also in tests in which blastospores were pre-treated with the drugs for 60 min, but was not detected in tests in which neutrophils were pre-treated for 60 min. Neither formulation affected neutrophil killing of C. albicans blastospores following simultaneous addition of the drug with the blastospores. However, previous treatment of the neutrophils with either formulation at a concentration of 20 mg/L led to enhanced killing of ingested blastospores. The results suggest that much higher concentrations of liposomal amphotericin B (AmBisome) are required to produce deleterious effects on neutrophil phagocytic function than with the conventional formulation of the drug.
脂质体两性霉素B（安必素）浓度≥20mg/L时，以及两性霉素B-脱氧胆酸盐（DC）（两性霉素B）浓度≥1mg/L时，在与芽生孢子同时添加药物后，两者均导致中性粒细胞对白色念珠菌芽生孢子的摄取显著减少。在芽生孢子用药物预处理60分钟的试验中也观察到摄取减少，但在中性粒细胞预处理60分钟的试验中未检测到。在与芽生孢子同时添加药物后，两种制剂均不影响中性粒细胞对白色念珠菌芽生孢子的杀伤。然而，用浓度为20mg/L的任何一种制剂预先处理中性粒细胞会导致对摄入芽生孢子的杀伤增强。结果表明，与传统制剂相比，需要更高浓度的脂质体两性霉素B（安必素）才能对中性粒细胞吞噬功能产生有害影响。
