Mehta R T, Poddar S, Kalidas M, Gomez-Flores R, Dulski K
Department of Bio-immunotherapy, University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.
J Infect Dis. 1997 Jan;175(1):214-7. doi: 10.1093/infdis/175.1.214.
The role of macrophage activation in the candidacidal activity of liposome-incorporated (L) amphotericin B was investigated. Macrophages treated with L-amphotericin B killed Candida albicans more effectively than did macrophages treated with free (F) amphotericin B. However, macrophages treated with neither F- nor L-amphotericin B killed amphotericin B-resistant Candida tropicalis. In vivo stimulation of macrophages by intraperitoneal administration of thioglycollate, Freund's complete adjuvant, or heat-killed C. albicans followed by in vitro treatment with F- or L-amphotericin B, did not enhance their candidacidal activity. Intravenous administration of F- or L-amphotericin B did not augment the candidacidal activity of macrophages sensitized in vivo; however, sensitized macrophages showed enhanced killing compared with resident unstimulated cells. These studies suggest that macrophage-mediated enhancement of C. albicans killing may be due to uptake, transport, and delivery of L-amphotericin B to infected sites rather than to macrophage activation.
研究了巨噬细胞活化在脂质体包裹（L）两性霉素B杀念珠菌活性中的作用。用L-两性霉素B处理的巨噬细胞比用游离（F）两性霉素B处理的巨噬细胞更有效地杀死白色念珠菌。然而，既未用F-也未用L-两性霉素B处理的巨噬细胞无法杀死对两性霉素B耐药的热带念珠菌。通过腹腔注射巯基乙酸盐、弗氏完全佐剂或热灭活的白色念珠菌对巨噬细胞进行体内刺激，随后用F-或L-两性霉素B进行体外处理，并未增强其杀念珠菌活性。静脉注射F-或L-两性霉素B并未增强体内致敏巨噬细胞的杀念珠菌活性；然而，与未受刺激的驻留细胞相比，致敏巨噬细胞的杀伤能力有所增强。这些研究表明，巨噬细胞介导的白色念珠菌杀伤增强可能是由于L-两性霉素B被摄取、转运并递送至感染部位，而非巨噬细胞活化所致。
