Kalashnikova E E, Chernyshova M P, Ignatov V V
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015 Russia.
Mikrobiologiia. 2003 Jul-Aug;72(4):498-502.
The culture liquids of three Xanthomonas campestris pv. campestris strains were found to possess proteolytic activity. The culture liquid of strain B-611 with the highest proteolytic activity was fractionated by salting-out with ammonium sulfate, gel filtration, and ion-exchange chromatography. The electrophoretic analysis of active fractions showed the presence of two proteases in the culture liquid of strain B-611, the major of which being serine protease. The treatment of cabbage seedlings with the proteases augmented the activity of peroxidase in the cabbage roots by 28%.
研究发现，三种野油菜黄单胞菌野油菜致病变种菌株的培养液具有蛋白水解活性。对蛋白水解活性最高的B-611菌株的培养液依次进行硫酸铵盐析、凝胶过滤和离子交换色谱分离。活性组分的电泳分析表明，B-611菌株的培养液中存在两种蛋白酶，其中主要的是丝氨酸蛋白酶。用这些蛋白酶处理甘蓝幼苗后，甘蓝根部过氧化物酶的活性增强了28%。
