Vijayaraghavan Ponnuswamy, Arasu Mariadhas Valan, Anantha Rajan R, Al-Dhabi N A
Bioprocess Engineering Division, Smykon Biotech Pvt Ltd, Nagercoil, Kanyakumari District, Tamil Nadu, India.
Addiriyah Chair for Environmental Studies, Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Saudi J Biol Sci. 2019 Feb;26(2):217-224. doi: 10.1016/j.sjbs.2018.08.029. Epub 2018 Sep 1.
Cardiovascular diseases (CVDs) cause high mortality throughout the world. Existing fibrinolytic agents are highly expensive and have many side effects. Microbial fibrinolytic enzymes are very much considered as novel therapeutic candidate for the treatment of CVDs. Reports on fibrinolytic enzyme from sp. is lacking. This study reports fibrinolytic enzymes from IND3 as it shows hyperactivity on fibrin-agarose plates. This organism utilized various agro-industrial wastes for enzymes production. Among all, cow dung enhanced more enzyme production, hence it was used as the low-cost substrate for statistical optimization of fibrinolytic protease in Solid state fermentation. Response surface methodology was employed to optimize the factors and enhanced yield by 4-fold. The interactions among the variables, viz, sucrose, yeast extract, and pH of the medium were investigated using Central Composite Design (CCD). The predicted fibrinolytic enzyme activity was 2340 U/g, and the observed fibrinolytic enzyme activity was 2294 ± 12.8 U/g. The fibrinolytic enzyme degraded blood clot completely. This study is the first report on statistical optimization of fibrinolytic enzyme production in SSF from sp. The crude extract has immense activity on proteinaceous wastes. The production of fibrinolytic protease using the low-cost substrate could reduce the production cost of enzyme.
心血管疾病（CVDs）在全球范围内导致高死亡率。现有的纤维蛋白溶解剂价格高昂且有许多副作用。微生物纤维蛋白溶解酶被广泛认为是治疗心血管疾病的新型治疗候选物。关于来自某菌株的纤维蛋白溶解酶的报道较少。本研究报道了来自IND3的纤维蛋白溶解酶，因为它在纤维蛋白 - 琼脂糖平板上表现出高活性。该微生物利用各种农业工业废料生产酶。其中，牛粪能提高更多的酶产量，因此它被用作固态发酵中纤维蛋白溶解蛋白酶统计优化的低成本底物。采用响应面法优化因素，并使产量提高了4倍。使用中心复合设计（CCD）研究了变量之间的相互作用，即蔗糖、酵母提取物和培养基的pH值。预测的纤维蛋白溶解酶活性为2340 U/g，观察到的纤维蛋白溶解酶活性为2294±12.8 U/g。该纤维蛋白溶解酶能完全降解血凝块。本研究是关于某菌株固态发酵中纤维蛋白溶解酶生产统计优化的首次报道。粗提物对蛋白质类废料具有巨大活性。使用低成本底物生产纤维蛋白溶解蛋白酶可以降低酶的生产成本。
