采用聚合酶链反应检测艾滋病患者脑脊液中的弓形虫。
Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by polymerase chain reaction.
作者信息
Parmley S F, Goebel F D, Remington J S
机构信息
Department of Immunology and Infectious Diseases, Research Institute, Palo Alto, California 94301.
出版信息
J Clin Microbiol. 1992 Nov;30(11):3000-2. doi: 10.1128/jcm.30.11.3000-3002.1992.
Abstract
The polymerase chain reaction (PCR) was used to detect Toxoplasma gondii DNA in cerebrospinal fluid (CSF) from 14 patients with AIDS by amplification of the repetitive B1 gene. Positive PCRs were obtained in CSF from four of nine patients with toxoplasmic encephalitis. CSF samples from five control patients were negative for T. gondii DNA by PCR.
摘要
采用聚合酶链反应（PCR），通过扩增重复的B1基因来检测14例艾滋病患者脑脊液（CSF）中的弓形虫DNA。9例弓形虫脑炎患者中有4例脑脊液PCR检测呈阳性。5例对照患者的脑脊液样本经PCR检测，弓形虫DNA呈阴性。
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本文引用的文献
1
Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction.通过聚合酶链反应直接灵敏地检测致病原生动物刚地弓形虫。
J Clin Microbiol. 1989 Aug;27(8):1787-92. doi: 10.1128/jcm.27.8.1787-1792.1989.
2
Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid.利用聚合酶链反应和羊水进行先天性弓形虫感染的快速产前诊断。
J Clin Microbiol. 1990 Oct;28(10):2297-301. doi: 10.1128/jcm.28.10.2297-2301.1990.
3
Use of the polymerase chain reaction to detect DNA sequences specific to pathogenic treponemes in cerebrospinal fluid.利用聚合酶链反应检测脑脊液中致病性密螺旋体特有的DNA序列。
FEMS Microbiol Lett. 1990 Mar 15;56(3):233-8. doi: 10.1111/j.1574-6968.1990.tb13943.x.
4
Sensitive and specific detection of toxoplasma DNA in an experimental murine model: use of Toxoplasma gondii-specific cDNA and the polymerase chain reaction.在实验性小鼠模型中灵敏且特异的弓形虫DNA检测：刚地弓形虫特异性cDNA及聚合酶链反应的应用
J Infect Dis. 1991 Jan;163(1):180-6. doi: 10.1093/infdis/163.1.180.
5
Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis.单纯疱疹性脑炎患者脑脊液中单纯疱疹病毒DNA的快速检测
Lancet. 1990 Feb 24;335(8687):440-1. doi: 10.1016/0140-6736(90)90667-t.
6
Identification of Toxoplasma gondii infections by BI gene amplification.通过BI基因扩增鉴定弓形虫感染
J Clin Microbiol. 1991 Oct;29(10):2120-4. doi: 10.1128/jcm.29.10.2120-2124.1991.
7
8
Detection of Toxoplasma gondii DNA by polymerase chain reaction in cerebrospinal fluid from AIDS patients with cerebral toxoplasmosis.采用聚合酶链反应检测艾滋病合并脑弓形虫病患者脑脊液中的弓形虫DNA。
J Infect Dis. 1992 May;165(5):982-3. doi: 10.1093/infdis/165.5.982.
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