单纯疱疹性脑炎患者脑脊液中单纯疱疹病毒DNA的快速检测
Rapid detection of herpes-simplex-virus DNA in cerebrospinal fluid of patients with herpes simplex encephalitis.
作者信息
Rowley A H, Whitley R J, Lakeman F D, Wolinsky S M
机构信息
Department of Pediatrics, Northwestern University, Chicago, Illinois.
出版信息
Lancet. 1990 Feb 24;335(8687):440-1. doi: 10.1016/0140-6736(90)90667-t.
Herpes-simplex-virus (HSV) DNA in cerebrospinal fluid was amplified by use of the polymerase chain reaction and identified by hybridisation to a specific oligonucleotide probe. Specimens of cerebrospinal fluid (CSF) from 4 of 4 patients with herpes simplex encephalitis were positive for HSV DNA, whereas CSF specimens from 6 patients with other central-nervous-system infections were negative. This technique may expedite diagnosis of herpes simplex encephalitis.
采用聚合酶链反应扩增脑脊液中的单纯疱疹病毒（HSV）DNA，并通过与特定寡核苷酸探针杂交进行鉴定。4例单纯疱疹性脑炎患者的脑脊液标本中，有4例HSV DNA呈阳性，而6例其他中枢神经系统感染患者的脑脊液标本呈阴性。该技术可能会加快单纯疱疹性脑炎的诊断。
Zotero 插件