Duchaud Eric, Rusniok Christophe, Frangeul Lionel, Buchrieser Carmen, Givaudan Alain, Taourit Séad, Bocs Stéphanie, Boursaux-Eude Caroline, Chandler Michael, Charles Jean-François, Dassa Elie, Derose Richard, Derzelle Sylviane, Freyssinet Georges, Gaudriault Sophie, Médigue Claudine, Lanois Anne, Powell Kerrie, Siguier Patricia, Vincent Rachel, Wingate Vincent, Zouine Mohamed, Glaser Philippe, Boemare Noël, Danchin Antoine, Kunst Frank
Laboratoire de Génomique des Microorganismes Pathogènes, Institut Pasteur, 28 rue du Dr. Roux, 75724 Paris Cedex 15, France.
Nat Biotechnol. 2003 Nov;21(11):1307-13. doi: 10.1038/nbt886. Epub 2003 Oct 5.
Photorhabdus luminescens is a symbiont of nematodes and a broad-spectrum insect pathogen. The complete genome sequence of strain TT01 is 5,688,987 base pairs (bp) long and contains 4,839 predicted protein-coding genes. Strikingly, it encodes a large number of adhesins, toxins, hemolysins, proteases and lipases, and contains a wide array of antibiotic synthesizing genes. These proteins are likely to play a role in the elimination of competitors, host colonization, invasion and bioconversion of the insect cadaver, making P. luminescens a promising model for the study of symbiosis and host-pathogen interactions. Comparison with the genomes of related bacteria reveals the acquisition of virulence factors by extensive horizontal transfer and provides clues about the evolution of an insect pathogen. Moreover, newly identified insecticidal proteins may be effective alternatives for the control of insect pests.
发光杆菌是线虫的共生体，也是一种广谱昆虫病原体。菌株TT01的完整基因组序列长度为5,688,987个碱基对（bp），包含4,839个预测的蛋白质编码基因。引人注目的是，它编码大量粘附素、毒素、溶血素、蛋白酶和脂肪酶，并含有多种抗生素合成基因。这些蛋白质可能在消除竞争者、宿主定殖、昆虫尸体的入侵和生物转化中发挥作用，使发光杆菌成为共生和宿主-病原体相互作用研究的一个有前途的模型。与相关细菌的基因组比较揭示了通过广泛的水平转移获得毒力因子，并为昆虫病原体的进化提供了线索。此外，新鉴定的杀虫蛋白可能是控制害虫的有效替代品。
