Mukherjee D P, Tunkle A S, Roberts R A, Clavenna A, Rogers S, Smith D
LSU Health Sciences Center, Shreveport, Louisiana 71130-3932, USA.
J Biomed Mater Res B Appl Biomater. 2003 Oct 15;67(1):603-9. doi: 10.1002/jbm.b.10050.
The objective of this study was to develop a synthetic bone graft in a paste form. Reported here are the results of the evaluation of a paste of chitosan glutamate (Protosan) and hydroxyapatite (referred to as a paste) used in a critical size defect model in rats. Eight-millimeter--diameter cranial defects were made in rat calvaria following a protocol approved by the animal review committee. Five groups were studied: (1) empty control, (2) defect filled with paste only, (3) defect filled with the paste containing bone-marrow aspirate, (4) defect filled with paste containing BMP-2, and (5) defect filled with paste containing osteoblasts cultured from bone-marrow aspirate. The sacrifice intervals were 9 and 18 weeks. Calvaria containing the defect were harvested, and the bone mineral density (BMD) was determined by dual energy X-ray absorptiometry. Push-out strength measurements were also performed. The BMD values of empty control were significantly lower than those of other groups at both 9 and 18 weeks. The mechanical properties, that is, push-out strengths and area under the push-out load and displacement were not significantly different between the samples. Histological examination of Goldner-trichromestained undecalcified sections showed the presence of mineralized bone spicules in the defect areas that were more prominent in those filled with paste and osteoblasts cultured from bone-marrow aspirate. Hence, this study demonstrated that the paste of chitosan glutamate and hydroxyapatite-containing osteoblasts cultured from bone-marrow aspirate would be an effective material to repair bone defects.
本研究的目的是开发一种膏状合成骨移植材料。本文报道了对壳聚糖谷氨酸盐（Protosan）和羟基磷灰石糊剂（以下简称糊剂）在大鼠临界尺寸骨缺损模型中的评估结果。在动物审查委员会批准的方案下，在大鼠颅骨上制造直径为8毫米的颅骨缺损。研究了五组：（1）空白对照组，（2）仅用糊剂填充缺损，（3）用含骨髓抽吸物的糊剂填充缺损，（4）用含BMP-2的糊剂填充缺损，（5）用含从骨髓抽吸物培养的成骨细胞的糊剂填充缺损。处死间隔为9周和18周。收集含缺损的颅骨，并用双能X线吸收法测定骨矿物质密度（BMD）。还进行了推出强度测量。在9周和18周时，空白对照组的BMD值均显著低于其他组。样品之间的力学性能，即推出强度以及推出载荷和位移曲线下的面积没有显著差异。对Goldner三色染色的未脱钙切片进行组织学检查，结果显示缺损区域存在矿化骨针，在填充有糊剂和从骨髓抽吸物培养的成骨细胞的缺损区域中更为明显。因此，本研究表明，含壳聚糖谷氨酸盐和从骨髓抽吸物培养的成骨细胞的羟基磷灰石糊剂将是修复骨缺损的有效材料。
