Murugan R, Ramakrishna S
Nanobioengineering Laboratory, Division of Bioengineering, Department of Mechanical Engineering, NUS Nanoscience & Nanotechnology Initiative, National University of Singapore, 9 Engineering Drive 1, Singapore 117576, Singapore.
Biomaterials. 2004 Aug;25(17):3829-35. doi: 10.1016/j.biomaterials.2003.10.016.
Aim of this study concerns to the development of bioresorbable composite materials for bone repair and regeneration. Despite nano hydroxyapatite (HA) has wide range of medical applications, particles mobilization and slow resorbable nature limits its use in certain applications particularly, periodontal and alveolar ridge augmentation. To enhance its usage, we have prepared HA composite bone paste with a natural polysaccharide, chitosan, using wet chemical method at low temperature. The prepared composites were analyzed by various physicochemical methods and suggesting that the nano HA crystallites are well intact with the chitosan macromolecules. FT-IR results are indicating the existence of hydroxyl and amide groups in addition to the characteristic peaks of nano HA in the composite paste. The physical nature of paste form implies that it would be highly beneficial for the particle immobilization upon implantation. In vitro physiological stability and solubility of the composite was performed in phosphate buffered saline under physiological condition and found that the rate of resorbability of composite was quite higher than nano HA. These findings suggest that the HA/chitosan composites may have a great impact on human health care systems as bioresorbable bone substitute.
本研究的目的是开发用于骨修复和再生的生物可吸收复合材料。尽管纳米羟基磷灰石（HA）具有广泛的医学应用，但颗粒的移动性和缓慢的可吸收性限制了其在某些应用中的使用，特别是在牙周和牙槽嵴增高方面。为了扩大其用途，我们采用低温湿化学方法，用天然多糖壳聚糖制备了HA复合骨糊剂。通过各种物理化学方法对制备的复合材料进行了分析，结果表明纳米HA微晶与壳聚糖大分子完好结合。傅里叶变换红外光谱（FT-IR）结果表明，复合糊剂中除了纳米HA的特征峰外，还存在羟基和酰胺基团。糊剂形式的物理性质表明，植入后对颗粒固定非常有利。在生理条件下，在磷酸盐缓冲盐水中对复合材料进行了体外生理稳定性和溶解性测试，发现复合材料的可吸收速率远高于纳米HA。这些发现表明，HA/壳聚糖复合材料作为生物可吸收骨替代物，可能对人类医疗保健系统产生重大影响。
