Esteve-Zarzoso Braulio, Zorman Tina, Belloch Carmela, Querol Amparo
Departamento de Biotecnologia, Instituto de Agroquimica y Tecnologia de Alimentos (CSIC), Burjassot, Valincia, Spain.
Syst Appl Microbiol. 2003 Sep;26(3):404-11. doi: 10.1078/072320203322497437.
The restriction fragments polymorphisms of the mitochondrial DNA and the PCR fragment that comprised the internal transcribes spacers and the 5.8S rRNA gene, together with the electrophoretic karyotypes of 40 strains from the 10 species of the genus Zygosaccharomyces, including the new species Z. lentus were examined. The RFLP's of the ITS-5.8S region showed a specific restriction pattern for each species, including the new species Z. lentus. The only exception were the species Z. cidri and Z. fermentati that produced identical restriction profiles. The electrophoretic chromosome patterns confirmed the differences between the species of this genus, including the phylogenetic closest species Z. cidri and Z. fermentati. They present few chromosomes ranging from 3 bands (4 or 5 chromosomes) for Z. florentinus to 7 bands (8 to 10 chromosomes) for Z. cidri and Z. fermentati. The strain level resolution power of RFLP's of mtDNA of this genus enabled the characterisation of strains from the same species, even where they are isolated from the same substrate. However, in the cases of Z. bailii and Z. lentus, electrophoretic karyotyping there was considerable variation.
对包括新种迟缓接合酵母（Z. lentus）在内的接合酵母属10个种的40个菌株进行了线粒体DNA的限制性片段多态性分析，以及包含内部转录间隔区和5.8S rRNA基因的PCR片段分析，并对其进行了电泳核型分析。ITS-5.8S区域的RFLP分析显示，每个种，包括新种迟缓接合酵母（Z. lentus），都有特定的限制性图谱。唯一的例外是西德里接合酵母（Z. cidri）和发酵接合酵母（Z. fermentati），它们产生相同的限制性图谱。电泳染色体图谱证实了该属不同种之间的差异，包括亲缘关系最近的西德里接合酵母（Z. cidri）和发酵接合酵母（Z. fermentati）。它们的染色体数量很少，弗洛伦廷接合酵母（Z. florentinus）有3条带（4或5条染色体），而西德里接合酵母（Z. cidri）和发酵接合酵母（Z. fermentati）有7条带（8至10条染色体）。该属线粒体DNA的RFLP分析在菌株水平上的分辨能力，能够对来自同一物种的菌株进行特征鉴定，即使它们是从同一底物中分离出来的。然而，在拜氏接合酵母（Z. bailii）和迟缓接合酵母（Z. lentus）的情况下，电泳核型分析存在相当大的差异。
