Bugnon C, Fellmann D, Lenys D, Bloch B
C R Seances Soc Biol Fil. 1977;171(4):907-13.
On paraffin or semi-thin sections various anti-LH or anti-TSH sera stain indifferently all the thyrotrophs and the gonadotrophs. Inversely anti-beta-TSH, anti-beta-LH or anti-beta-FSH purified sera permit the discrimination of these two cell populations. The constancy of fixation of the anti-beta-LH and anti-beta-FSH sera on all the gonadotrophs gives evidence of their ability to produce both FSH and LH. However in a few female rats the central gonadotrophs are stained more weakly by anti-beta-FSH serum than by anti-beta-LH serum. The purification of the antisera by adjunction of hormonal antigens (alpha subunits or heterologous hormone) does not enable, with the PAP technique on thin sections, a selective staining of the secretory granules of the thyrotrophs or of the gonadotrophs.
在石蜡切片或半薄切片上，各种抗促黄体生成素（LH）或抗促甲状腺激素（TSH）血清可无差别地染色所有促甲状腺细胞和促性腺细胞。相反，抗β-TSH、抗β-LH或抗β-促卵泡激素（FSH）纯化血清可区分这两种细胞群体。抗β-LH和抗β-FSH血清在所有促性腺细胞上固定的一致性证明了它们产生FSH和LH的能力。然而，在少数雌性大鼠中，中央促性腺细胞被抗β-FSH血清染色比被抗β-LH血清染色更弱。通过添加激素抗原（α亚基或异源激素）对抗血清进行纯化，在薄切片上采用过氧化物酶-抗过氧化物酶（PAP）技术，无法对促甲状腺细胞或促性腺细胞的分泌颗粒进行选择性染色。
