Zhou Z X, Deng Z P, Chen J L
Department of Histology and Embryology, College of Veterinary Medicine, Beijing Agricultural University, People's Republic of China.
J Reprod Fertil. 1994 Mar;100(2):417-23. doi: 10.1530/jrf.0.1000417.
Lectin-binding glycoconjugates in the endometrium of miniature pigs were determined for sexually immature, dioestrus, oestrus and early pregnancy periods, using five horseradish peroxidase-labelled lectins. Apparent changes associated with sexual maturity were increases in binding intensity of wheat germ agglutinin (WGA), Glycine max agglutinin (SBA) and Ulex europaeus agglutinin (UEA) and the appearance of Ricinus communis agglutinin (RCA) binding. The binding intensity of WGA, SBA and UEA further increased at oestrus, particularly for UEA which showed a stage-specific binding on the surface of endometrial epithelium. Pregnancy-related changes included an increase in WGA binding and a decrease in UEA and RCA binding. The results indicate that stage-specific alterations in lectin binding glycoconjugates occur in the endometrium of pigs associated with sexual maturity and the reproductive cycle.
使用五种辣根过氧化物酶标记的凝集素，测定了小型猪子宫内膜在性未成熟、发情间期、发情期和妊娠早期的凝集素结合糖缀合物。与性成熟相关的明显变化是麦胚凝集素（WGA）、大豆凝集素（SBA）和荆豆凝集素（UEA）的结合强度增加，以及蓖麻凝集素（RCA）结合的出现。WGA、SBA和UEA的结合强度在发情期进一步增加，特别是UEA在子宫内膜上皮表面表现出阶段特异性结合。与妊娠相关的变化包括WGA结合增加以及UEA和RCA结合减少。结果表明，猪子宫内膜中凝集素结合糖缀合物的阶段特异性改变与性成熟和生殖周期有关。
