通过在根尖上培养来拯救菊花茎尖分生组织。
Rescue of shoot apical meristems of chrysanthemum by culturing on root tips.
作者信息
Hosokawa M, Otake A, Sugawara Y, Hayashi T, Yazawa S
机构信息
Laboratory of Vegetable and Ornamental Horticulture, Department of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Sakyo-ku, 606-8502, Kyoto, Japan.
出版信息
Plant Cell Rep. 2004 Feb;22(7):443-8. doi: 10.1007/s00299-003-0719-1. Epub 2003 Oct 3.
A new method to regenerate plants from leaf primordia-free shoot apical meristem domes (LP-free SAMs) was developed by establishing the meristem dome on the cut surface of root tips. Ten days after culture, the viable rate of LP-free SAMs of chrysanthemum 'Piato' attached to chrysanthemum root tips was >40%. Shoot regeneration was not observed from LP-free SAMs without the root tips. When LP-free SAMs of chrysanthemum were transferred to root tips of either petunia, cabbage, or carnation, the highest shoot regeneration rate was observed with cabbage root tips. Microscopic observation documented that the LP-free SAM temporarily adhered to the cut surface of the root tip of cabbage.
通过在根尖切割面上建立分生组织穹顶，开发了一种从无叶原基茎尖分生组织穹顶（LP-free SAMs）再生植株的新方法。培养10天后，附着在菊花根尖上的菊花“Piato”的LP-free SAMs的成活率>40%。没有根尖的LP-free SAMs未观察到芽再生。当菊花的LP-free SAMs转移到矮牵牛、卷心菜或康乃馨的根尖上时，用卷心菜根尖观察到最高的芽再生率。显微镜观察记录了LP-free SAMs暂时附着在卷心菜根尖的切割面上。
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