Nencheva D
Institute of Ornamental Plants, Agricultural Academy, Sofia, Bulgaria.
Methods Mol Biol. 2010;589:177-85. doi: 10.1007/978-1-60327-114-1_17.
Chrysanthemum flowers Chrysanthemum x grandiflorum (Ramat.) Kitam., are commercially significant worldwide as there are large number of cultivars for cut flowers, pot flowers, and garden flowers. Commercial in vitro multiplication of chrysanthemum is often based on stem nodal explants with lateral meristems. This chapter describes a protocol for in vitro propagation from stem nodal explants and by direct organogenesis from pedicel explants producing large number of true-to-type plantlets in 4-8 week on Murashige and Skoog (MS) based media. Also, true mutants with changed flower color are obtained without producing chimeras after gamma-irradiation in mutation breeding.
菊花（Chrysanthemum x grandiflorum (Ramat.) Kitam.）在全球商业上具有重要意义，因为有大量用于切花、盆花和园林花卉的品种。菊花的商业离体繁殖通常基于带有侧生分生组织的茎节外植体。本章描述了一种基于Murashige和Skoog（MS）培养基，从茎节外植体进行离体繁殖以及通过花梗外植体直接器官发生的方案，该方案能在4 - 8周内产生大量与母本一致的植株。此外，在诱变育种中，经伽马射线辐照后可获得花色改变的真实突变体，且不会产生嵌合体。
