Kikuchi H, Sakata T
Yakult Central Institute for Microbiological Research, Tokyo, Japan.
J Nutr Sci Vitaminol (Tokyo). 1992 Jun;38(3):287-96. doi: 10.3177/jnsv.38.287.
Pectin, guar gum, transgalactosylated oligosaccharide and glucose were estimated as substrate for hindgut fermentation by measuring the volume of gas released from a 1 ml scale batch culture using rat cecal contents. The amount of short-chain fatty acids (SCFA) in cultures of glucose and pectin was also measured. The amount of SCFA (Y mumol/culture) or volume of gas released (Y ml/culture) was expressed as an exponential function of incubation time (t) (Y = A+B x (1-e-kt); A, B and k are constants). The amount of potential production of gas and SCFA (B) for glucose was higher than that for pectin and the rate constant of gas and SCFA (k) for pectin was higher than that for glucose. The volume of gas released (ml/culture) correlated positively and linearly with the amount of total SCFA (acetic, propionic and n-butyric acids, mumol/culture). A single regression stood for both glucose and pectin. The gas release followed saturation kinetics. The half maximal concentration and maximal velocity for gas release from pectin were higher than those from other substrates (glucose, guar gum and transgalactosylated oligosaccharide).
通过测量使用大鼠盲肠内容物的1毫升规模分批培养物释放的气体体积，评估果胶、瓜尔胶、转半乳糖基寡糖和葡萄糖作为后肠发酵底物的情况。还测量了葡萄糖和果胶培养物中短链脂肪酸（SCFA）的含量。SCFA的量（Y微摩尔/培养物）或释放的气体体积（Y毫升/培养物）表示为孵育时间（t）的指数函数（Y = A + B x (1 - e-kt)；A、B和k为常数）。葡萄糖的潜在产气量和SCFA量（B）高于果胶，果胶的产气和SCFA速率常数（k）高于葡萄糖。释放的气体体积（毫升/培养物）与总SCFA量（乙酸、丙酸和正丁酸，微摩尔/培养物）呈正线性相关。葡萄糖和果胶均符合一元回归。气体释放遵循饱和动力学。果胶释放气体的半数最大浓度和最大速度高于其他底物（葡萄糖、瓜尔胶和转半乳糖基寡糖）。
