Stewart M J, Vanderberg J P
Department of Medical and Molecular Parasitology, New York University School of Medicine, New York 10016.
J Protozool. 1992 Nov-Dec;39(6):663-71. doi: 10.1111/j.1550-7408.1992.tb04446.x.
Immunoelectron microscopic techniques were utilized to characterize the morphology of circumsporozoite protein-containing trails deposited on various substrates by gliding Plasmodium berghei and Plasmodium falciparum sporozoites. The basic components of the trails are beadlike particles, 25 to 90 nm in diameter, which are devoid of unit membrane and have an electron-lucent center. Trails were captured on formvar-covered grids coated with anticircumsporozoite protein monoclonal antibodies and compared with trails produced on uncoated formvar; the results suggest that material containing circumsporozoite protein forms the matrix within which the particles are embedded. The trails exhibit morphological features similar to those displayed by circumsporozoite precipitation reactions; of note is the demonstration of sheaths of circumsporozoite protein-containing material that emanate from sporozoites prior to their gliding. The sheaths narrow into accumulations of electron-dense material, which eventually taper to form typical trails. The structural manifestation of sheaths and other morphological details of the formed trails enables us to correlate sporozoite behavior during trail formation with the motile actions of gliding sporozoites observed by light microscopy.
利用免疫电子显微镜技术来表征伯氏疟原虫和恶性疟原虫子孢子滑行时沉积在各种底物上的含环子孢子蛋白痕迹的形态。这些痕迹的基本成分是直径为25至90纳米的珠状颗粒，它们没有单位膜且有一个电子透明中心。在涂有抗环子孢子蛋白单克隆抗体的福尔马林中涂覆的网格上捕获痕迹，并与在未涂覆的福尔马林中产生的痕迹进行比较；结果表明，含环子孢子蛋白的物质形成了颗粒嵌入其中的基质。这些痕迹表现出与环子孢子沉淀反应所显示的形态特征相似；值得注意的是，展示了在子孢子滑行之前从子孢子发出的含环子孢子蛋白物质的鞘。这些鞘变窄成电子致密物质的聚集物，最终逐渐变细形成典型的痕迹。鞘的结构表现和形成的痕迹的其他形态细节使我们能够将痕迹形成过程中的子孢子行为与光学显微镜观察到的滑行子孢子的运动行为联系起来。
