de Paula Adelzon A, da Silva Alba V M, Fernandes Octávio, Jansen Ana M
Laboratório de Biologia de Tripanosomatídeos, Instituto Oswaldo Cruz FIOCRUZ, Avenida Brasil 4365, Manguinhos, 21045-900 Rio de Janeiro, Brazil.
J Parasitol. 2003 Aug;89(4):832-6. doi: 10.1645/GE-2988.
In this article, we describe the findings obtained using immunoblot analysis in the diagnosis of canine visceral leishmaniasis (VL) and its correlation with serological titer and clinical status. We found that all animals bearing amastigote forms recognized antigens with 29 and 32 kDa and that this pattern can be exploited for diagnostic and epidemiological purposes. The recognition of the 29- and 32-kDa antigens was verified even in seronegative dogs and preceded seroconversion in periods ranging from several months to 2 yr. We found a correlation between serological titer and parasite burden. Although no correlation between antigenic recognition pattern and clinical status was observed, immunoblot analysis proved to be a reliable test to detect antibodies against Leishmania sp. antigens in dogs from areas with endemic VL.
在本文中，我们描述了使用免疫印迹分析诊断犬内脏利什曼病（VL）所获得的结果及其与血清学滴度和临床状况的相关性。我们发现，所有带有无鞭毛体形式的动物都能识别29 kDa和32 kDa的抗原，并且这种模式可用于诊断和流行病学目的。即使在血清阴性的犬中也证实了对29 kDa和32 kDa抗原的识别，并且在数月至2年的时间段内血清转化之前就已出现。我们发现血清学滴度与寄生虫负荷之间存在相关性。虽然未观察到抗原识别模式与临床状况之间的相关性，但免疫印迹分析被证明是检测来自VL流行地区犬中针对利什曼原虫属抗原抗体的可靠测试。
