Corredor Mauricio, Davila Anne-Marie, Casarégola Serge, Gaillardin Claude
Laboratoire de Génétique Moléculaire et Cellulaire INRA UMR216, CNRS URA 1925, Collection de Levures d'Intérêt Biotechnologique, Institut National Agronomique Paris-Grignon, 78850 Thiverval-Grignon, France.
Antonie Van Leeuwenhoek. 2003;84(2):81-8. doi: 10.1023/a:1025432721866.
Pulse field gel electrophoresis karyotypes of 41 strains of the genus Debaryomyces, including 35 strains confirmed as D. hansenii species by D1/D2 ribosomal DNA sequence analysis, were performed. Electrophoretic karyotypes of the 41 strains exhibited 4 to 10 chromosomal bands ranging between 0.7 Mb and 4.2 Mb. Among D. hansenii species, the patterns of strains obtained from the CBS collection and cheese isolates differed strongly from D. hansenii var. hansenii CBS767T. Both D. hansenii var. hansenii and D. hansenii var. fabryii showed chromosome length polymorphism. Electrophoretic karyotypes of the D. hansenii strains were analyzed by Southern hybridization with various species-specific probes isolated from D. hansenii var. hansenii CBS767T. Repeated sequences including the F01pro, M18pro, the Ty1-copia retrotransposon Tdh5 and hypothetical telomeric sequence hybridized to several chromosomal bands, while a D1/D2 probe derived from the large ribosomal sub-unit hybridized only to the largest chromosome. Unique probes such as those hybridizing to actin ACT1, glycerol-3-phosphate dehydrogenase GPD1 and beta-glucosidase LAC4 encoding genes were assigned to specific chromosomal bands of D. hansenii var. hansenii CBS767T. These probes failed to hybridize to D. hansenii var. fabryii strongly suggesting that strains of this variety actually represent a different taxon.
对41株德巴利酵母属菌株进行了脉冲场凝胶电泳核型分析，其中35株通过D1/D2核糖体DNA序列分析被确认为汉逊德巴利酵母菌种。41株菌株的电泳核型显示出4至10条染色体带，大小在0.7兆碱基对至4.2兆碱基对之间。在汉逊德巴利酵母菌种中，从CBS菌种保藏中心获得的菌株和奶酪分离株的模式与汉逊德巴利酵母变种CBS767T有很大不同。汉逊德巴利酵母变种和法布里汉逊德巴利酵母变种均表现出染色体长度多态性。用从汉逊德巴利酵母变种CBS767T分离的各种种特异性探针通过Southern杂交分析汉逊德巴利酵母菌株的电泳核型。包括F01pro、M18pro、Ty1-copia反转录转座子Tdh5和假定端粒序列在内的重复序列与几条染色体带杂交，而来自大核糖体亚基的D1/D2探针仅与最大的染色体杂交。与肌动蛋白ACT1、甘油-3-磷酸脱氢酶GPD1和β-葡萄糖苷酶LAC4编码基因杂交的独特探针被定位到汉逊德巴利酵母变种CBS767T的特定染色体带上。这些探针未能与法布里汉逊德巴利酵母变种强烈杂交，这强烈表明该变种的菌株实际上代表了一个不同的分类单元。
