Nguyen Huu-Vang, Gaillardin Claude, Neuvéglise Cécile
Collection de Levures d'Intérêt Biotechnologique, Laboratoire de Microbiologie et Génétique Moléculaire, Centre Versailles-Grignon, INRA (UMR1238) CNRS (UMR2585) AgroParisTech, Thiverval-Grignon, France.
FEMS Yeast Res. 2009 Jun;9(4):641-62. doi: 10.1111/j.1567-1364.2009.00510.x. Epub 2009 Apr 6.
The intergenic spacer rDNA amplification and AluI fingerprinting (IGSAF) method detected four distinct groups among 170 Debaryomyces hansenii strains: D. hansenii var. hansenii; Candida famata var. famata; D. hansenii var. fabryi and C. famata var. flareri. IGS sequence comparison of representative strains showed that D. hansenii var. hansenii and C. famata var. famata belonged to one species, whereas D. hansenii var. fabryi and C. famata var. flareri belonged to two different ones. This confirmed the following three species recently reinstated: D. hansenii (=C. famata), Debaryomyces fabryi and Debaryomyces subglobosus (=Candida flareri). Accordingly, growth at 37 degrees C may no longer be used to differentiate D. hansenii from D. fabryi. Riboflavin production is more specific for D. fabryi and D. subglobosus strains. IGSAF identified all the other 17 species of the genus Debaryomyces, six of them sharing with D. hansenii an rRNA gene unit harbouring two 5S rRNA genes. The phylogenetic tree established with IGS sequences was congruent with the one based on ACT1, GPD1 and COX2 sequences depicting a distinct D. hansenii clade close to the D. subglobosus, Debaryomyces prosopidis and D. fabryi clade. Description of Debaryomyces vietnamensis sp. nov. (type strain CBS 10535(T), MUCL 51648(T)), closely related to Debaryomyces nepalensis is given.
基因间隔区核糖体DNA扩增及AluI指纹图谱（IGSAF）方法在170株汉逊德巴利酵母菌株中检测到四个不同的组：汉逊德巴利酵母变种汉逊德巴利酵母；法马塔假丝酵母变种法马塔假丝酵母；汉逊德巴利酵母变种法布里酵母和法马塔假丝酵母变种弗拉雷假丝酵母。代表性菌株的IGS序列比较表明，汉逊德巴利酵母变种汉逊德巴利酵母和法马塔假丝酵母变种法马塔假丝酵母属于同一个物种，而汉逊德巴利酵母变种法布里酵母和法马塔假丝酵母变种弗拉雷假丝酵母属于两个不同的物种。这证实了最近恢复的以下三个物种：汉逊德巴利酵母（=法马塔假丝酵母）、法布里德巴利酵母和近球形德巴利酵母（=弗拉雷假丝酵母）。因此，37℃下的生长可能不再用于区分汉逊德巴利酵母和法布里德巴利酵母。核黄素的产生对法布里德巴利酵母和近球形德巴利酵母菌株更具特异性。IGSAF鉴定出了德巴利酵母属的所有其他17个物种，其中六个与汉逊德巴利酵母共享一个含有两个5S rRNA基因的rRNA基因单元。用IGS序列建立的系统发育树与基于ACT1、GPD1和COX2序列的系统发育树一致，描绘了一个与近球形德巴利酵母、人面德巴利酵母和法布里德巴利酵母分支接近的独特的汉逊德巴利酵母分支。描述了与尼泊尔德巴利酵母密切相关的越南德巴利酵母新种（模式菌株CBS 10535(T)，MUCL 51648(T)）。
